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Anti-human T lymphocyte serum specific to the receptor for sheep erythrocytes (E) was produced by immunizing sheep with autologous erythrocytes sensitized with solubilized receptors from human lymphocytes. The anti-soluble receptors serum (anti-Rs) inhibited E-Rosette formation, identified T lymphocytes by indirect immunofluorescence, agglutinated the formalized E-soluble receptors complexes, and inhibited the E-soluble receptors interaction. This anti-Rs serum was also submitted to the affinity chromatography by protein A sepharose, and the amounts of 0.15 to 120 micrograms of purified anti-Rs IgG were added to the lymphocyte culture. The result obtained showed an induction of proliferative response of the normal human lymphocytes in the presence of 60 and 120 micrograms of anti-Rs IgG.

Cell cultures treated with tylosin tartrate and kanamycin sulphate antibiotics were studied in relation to the cell growth rate, the susceptibility to the foot-and-mouth disease virus and to the microorganism eradication. These treatments did not affect the cell growth rate and the cell behavior to the viral infection. On the other hand, the decontamination of the intracytoplasmatic formas of mycoplasma-like organism was not observed.

Morphology and chromosomes of cell sublines derived from two new bovine kidney lines are reported. Cell susceptibility to the foot-and-mouth disease virus is discussed. One of the sublines showed epithelial-like cells, while the remainder, elonged fibroblastic-like cells. Most of them had a diploid number of chromosomes. From these sublines only one was sensible to the foot-and-mouth disease virus.