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Resumo O CAST (Caries Assessment Spectrum and Treatment) é um instrumento desenvolvido para a detecção de cárie a ser utilizado em levantamentos epidemiológicos. Foi validado e tem se mostrado efetivo, fornecendo um diagnóstico mais preciso do estado de saúde bucal do que o critério OMS, recomendado pela Organização Mundial da Saúde. O objetivo deste artigo é comparar a apresentação dos resultados de cárie dentária utilizando o instrumento CAST e o critério OMS, numa mesma população. Foram avaliados por dois examinadores treinados na utilização do instrumento CAST 680 escolares de 6 a 8 anos do Distrito Federal, Brasil. A avaliação constou do índice de placa visível (IPV) e do índice de sangramento gengival (ISG). Os escores CAST dente foram convertidos em componentes ceo/CPO e calculados os ceod/CPOD. Os pais responderam a um questionário sociodemográfico. A idade média foi 7,45 anos (± 0,91). A prevalência de cárie na dentição decídua foi de 65,44% e 61,61%, considerando o CAST e o critério da OMS, respectivamente; na dentição permanente: 38,19% e 10,2%, respectivamente. A média do ceod foi de 2.4 (± 2.7) e a média do CPOD 0.16 (± 0.53). o IPV foi associado a maiores CAST máximos p < 0,005. O instrumento CAST demonstrou maior sensibilidade em identificar a presença e gravidade de lesões cariosas quando comparado ao critério OMS. Abstract Caries Assessment Spectrum and Treatment (CAST) is an instrument developed for caries detection to be used in epidemiological surveys; it has been validated and is believed to provide a clearer picture of the oral health status than the criteria provided by the World Health Organization (WHO). This article aims to compare the epidemiological survey results using the CAST instrument and the WHO criteria in the same population. 680 schoolchildren aged 6-8 years from Federal District, Brazil, were evaluated by two examiners trained to use the CAST. The visible plaque index (VPI) and gingival bleeding index (GBI) were also evaluated. The maximum CAST codes per tooth were converted into the dmf/DMF, the mean scores for primary and permanent dentition were calculated. The mean age was 7.45(± 0.91) years. The prevalence of caries differed when CAST and the WHO criteria were applied. In the primary dentition, it was 65.44% and 61.61%, and for the permanent dentition, 38.19% and 10.2%, respectively. It was possible to calculate the mean dmft [2.4(± 2.7)] and the DMFT [0.16(± 0.53)] using CAST. VPI was associated with higher maximum CAST scores p < 0,005. The way CAST results are presented showed a higher sensibility to identify the presence and severity of carious lesions in comparison to the WHO criteria.

Financiadora de Estudos e Projetos Epigenetics can be defined as the study of heritable changes in phenotype without the occurrence of changes in nucleotide sequence. Epigenetic modifications occur by the chemical changes in DNA and their associated proteins, such as DNA methylation and histone acetylation, respectively. Meat tenderness is a trait of great interest worldwide, resulting in the development of the beef livestock sector to produce meat of quality. It is worth to highlight the role of the calpain/calpastatin system on tenderness. Calpain encoded by the CAPN1 gene plays a role in proteolysis posmortem by cleaving proteins from muscle fiber. The calpastatin encoded by the CAST gene, on the other hand, acts controlling this cleavage by blocking the action of calpain. In addition, this system is also involved in myoblast differentiation into myotubes during embryogenesis. This system controls the proteolysis of proteins that constitute the cytoskeleton and the plasma membrane. To mimic the myotube formation during embryogenesis and to study the epigenetic control of CAPN1 and CAST gene expression, satellite cell cultures established from bovine muscle were kept undifferentiated (negative control) or were induced to differentiate by incubation with culture medium containing 2% fetal bovine serum in the absence (positive control) or after treatment with epigenetic modifiers like 5-Aza- 2'-deoxycytidine (Aza, DNA demethylating) for 48 h at 10 μM, and Trichostatin A (TSA, histone acetylating) for 24 h at 50 nM. The results showed no differences (p>0.05) in myoblast rate fusion between Aza, TSA and positive control groups, but there were differences (p>0.05) when these groups were compared to the negative control, which showed lower fusion rates. There was no difference (p>0.05) in cell viability among the four groups, showing that Aza and TSA were not cytotoxic at the used concentrations. Concerning the gene expression, the gene CAST was more expressed (p0,05) between positive control, 5-Aza-2 - deoxycytidine and Trichostatin A groups. For the CAPN1 gene, no difference was seen in the expression (p>0,05) between negative and positive control groups, but the CAPN1 gene was more expressed (p0.05) in myoblast rate fusion between Aza, TSA and positive control groups, but there were differences (p>0.05) when these groups were compared to the negative control, which showed lower fusion rates. There was no difference (p>0.05) in cell viability among the four groups, showing that Aza and TSA were not cytotoxic at the used concentrations. Concerning the gene expression, the gene CAST was more expressed (p0,05) between positive control, 5-Aza-2 - deoxycytidine and Trichostatin A groups. For the CAPN1 gene, no difference was seen in the expression (p>0,05) between negative and positive control groups, but the CAPN1 gene was more expressed (p0.05) in myoblast rate fusion between Aza, TSA and positive control groups, but there were differences (p>0.05) when these groups were compared to the negative control, which showed lower fusion rates. There was no difference (p>0.05) in cell viability among the four groups, showing that Aza and TSA were not cytotoxic at the used concentrations. Concerning the gene expression, the gene CAST was more expressed (p0,05) between positive control, 5-Aza-2 - deoxycytidine and Trichostatin A groups. For the CAPN1 gene, no difference was seen in the expression (p>0,05) between negative and positive control groups, but the CAPN1 gene was more expressed (p0,05) no índice de fusão dos mioblastos entre os grupos Aza, TSA e controle positivo, mas que houve diferença (p0,05) nas taxas de viabilidade das células entre os grupos, mostrando que o Aza e TSA não foram citotóxicos nas concentrações usadas Em relação à expressão gênica, o gene CAST foi mais expresso (p0,05) entre os grupos controle positivo, 5-Aza-2 -desoxicitidina e Tricostatina A. Para o gene CAPN1, não foi observada diferença (p>0,05) de expressão entre os grupos controle negativo e positivo, mas o gene CAPN1 foi mais expresso (p0,05) no índice de fusão dos mioblastos entre os grupos Aza, TSA e controle positivo, mas que houve diferença (p0,05) nas taxas de viabilidade das células entre os grupos, mostrando que o Aza e TSA não foram citotóxicos nas concentrações usadas Em relação à expressão gênica, o gene CAST foi mais expresso (p0,05) entre os grupos controle positivo, 5-Aza-2 -desoxicitidina e Tricostatina A. Para o gene CAPN1, não foi observada diferença (p>0,05) de expressão entre os grupos controle negativo e positivo, mas o gene CAPN1 foi mais expresso (p0,05) no índice de fusão dos mioblastos entre os grupos Aza, TSA e controle positivo, mas que houve diferença (p0,05) nas taxas de viabilidade das células entre os grupos, mostrando que o Aza e TSA não foram citotóxicos nas concentrações usadas Em relação à expressão gênica, o gene CAST foi mais expresso (p0,05) entre os grupos controle positivo, 5-Aza-2 -desoxicitidina e Tricostatina A. Para o gene CAPN1, não foi observada diferença (p>0,05) de expressão entre os grupos controle negativo e positivo, mas o gene CAPN1 foi mais expresso (p

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:02:04Z No. of bitstreams: 1 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:02:04Z No. of bitstreams: 1 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) Made available in DSpace on 2013-08-22T19:02:04Z (GMT). No. of bitstreams: 1 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) Previous issue date: 1995-12-01 Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:02:04Z No. of bitstreams: 1 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) Made available in DSpace on 2013-08-22T19:02:04Z (GMT). No. of bitstreams: 1 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) Previous issue date: 1995-12-01 Made available in DSpace on 2013-08-22T19:02:04Z (GMT). No. of bitstreams: 1 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) Previous issue date: 1995-12-01 Made available in DSpace on 2013-09-30T20:02:35Z (GMT). No. of bitstreams: 2 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) S0104-530X1995000300002.pdf.txt: 38902 bytes, checksum: 3d5f031f8610861336197b4885b54b0c (MD5) Previous issue date: 1995-12-01 Made available in DSpace on 2013-09-30T20:02:35Z (GMT). No. of bitstreams: 2 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) S0104-530X1995000300002.pdf.txt: 38902 bytes, checksum: 3d5f031f8610861336197b4885b54b0c (MD5) Previous issue date: 1995-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:16:02Z No. of bitstreams: 2 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) S0104-530X1995000300002.pdf.txt: 38902 bytes, checksum: 3d5f031f8610861336197b4885b54b0c (MD5) Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:16:02Z No. of bitstreams: 2 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) S0104-530X1995000300002.pdf.txt: 38902 bytes, checksum: 3d5f031f8610861336197b4885b54b0c (MD5) Made available in DSpace on 2013-08-22T19:02:04Z (GMT). No. of bitstreams: 1 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) Previous issue date: 1995-12-01 Made available in DSpace on 2013-09-30T20:02:35Z (GMT). No. of bitstreams: 2 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) S0104-530X1995000300002.pdf.txt: 38902 bytes, checksum: 3d5f031f8610861336197b4885b54b0c (MD5) Previous issue date: 1995-12-01 Made available in DSpace on 2014-05-20T15:16:02Z (GMT). No. of bitstreams: 2 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) S0104-530X1995000300002.pdf.txt: 38902 bytes, checksum: 3d5f031f8610861336197b4885b54b0c (MD5) Previous issue date: 1995-12-01 Made available in DSpace on 2014-05-20T15:16:02Z (GMT). No. of bitstreams: 2 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) S0104-530X1995000300002.pdf.txt: 38902 bytes, checksum: 3d5f031f8610861336197b4885b54b0c (MD5) Previous issue date: 1995-12-01 Made available in DSpace on 2013-09-30T20:02:35Z (GMT). No. of bitstreams: 2 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) S0104-530X1995000300002.pdf.txt: 38902 bytes, checksum: 3d5f031f8610861336197b4885b54b0c (MD5) Previous issue date: 1995-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:16:02Z No. of bitstreams: 2 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) S0104-530X1995000300002.pdf.txt: 38902 bytes, checksum: 3d5f031f8610861336197b4885b54b0c (MD5) Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:16:02Z No. of bitstreams: 2 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) S0104-530X1995000300002.pdf.txt: 38902 bytes, checksum: 3d5f031f8610861336197b4885b54b0c (MD5) Made available in DSpace on 2014-05-20T15:16:02Z (GMT). No. of bitstreams: 2 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) S0104-530X1995000300002.pdf.txt: 38902 bytes, checksum: 3d5f031f8610861336197b4885b54b0c (MD5) Previous issue date: 1995-12-01 Made available in DSpace on 2014-05-20T15:16:02Z (GMT). No. of bitstreams: 2 S0104-530X1995000300002.pdf: 226799 bytes, checksum: ef3128a6c7b4e1a7748104f46b9c82fd (MD5) S0104-530X1995000300002.pdf.txt: 38902 bytes, checksum: 3d5f031f8610861336197b4885b54b0c (MD5) Previous issue date: 1995-12-01 Este trabalho apresenta a modelagem de um problema particular de Programação da Produção numa Fundição Automatizada e sua resolução por um algoritmo de busca heurística, que explora a estrutura do problema. This paper presents a mathematical model of a particular Planning and Control Production Problem and its resolution using a heuristic search algorithm, which explores the structure of the problem. Universidade Estadual Paulista Departamento de Computação Universidade de São Paulo Departamento de Computação e Estatística Universidade Estadual Paulista Departamento de Computação

Este trabalho apresenta a modelagem de um problema particular de Programação da Produção numa Fundição Automatizada e sua resolução por um algoritmo de busca heurística, que explora a estrutura do problema. This paper presents a mathematical model of a particular Planning and Control Production Problem and its resolution using a heuristic search algorithm, which explores the structure of the problem.