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Background: Flu is one of the oldest medical concerns, causing high mortality rates among humans. Swine flu has not only emerged as a fatal disease omong Indian population but has also created havoc among various sections of society., Objective: To determine Knowledge, Attitude and Anxiety towards pandemic flu a potential bioweapon among health care professionals in Indore City., Method: The study design used was a cross sectional descriptive study was carried out between February-March 2015 during the outbreak of Swine Flu. The survey was administered to a sample of 271 health professionals. Participants comprised of 148 medical professionals and 123 dental professionals practicing in Sri Aurobindo Institute of Medical Science. The data collection tools comprised of a questionnaire on demographic characteristics, knowledge and attitude. Beck Anxiety Inventory was used to assess the anxiety among professionals., Results: The medical professionals (95.9%) had significantly higher (p value=0.007) knowledge about available vaccines against Swine Flu. The dental professionals (33.3%) were more hesistant in treating patients suffering from Swine Flu compared to medical., Conclusion: The results of the present study suggest that the health care professionals had good knowledge, showed positive attitude, and demonstrated lower anxiety levels.