1. [Use of acetocarmines for staining of larvae of digenetic Trematoda in situ].
- Author
-
Pokora Z and Szilman P
- Subjects
- Animals, Larva anatomy & histology, Larva cytology, Larva drug effects, Staining and Labeling methods, Trematoda cytology, Trematoda drug effects, Carmine analogs & derivatives, Trematoda anatomy & histology
- Abstract
Qualities of microscopical pictures of digeneic larvae total slides stained with different acetocarmines were compared. Semichon's acidifying carmine for topographical staining, Schneider's and Belling's carmines for chromosomes as well as iron acetocarmine (modification of the last two) were used. The last stain permits to obtain the slides, that in respect of their colour and quality of microscopical picture may be compared with slides stained with Semichon's carmine. Contrast, colour and reaction of staining depend on concentration of iron acetate in the staining solution. Total slides of investigated larvae stained respectively with Schneider's and Belling's carmines are not enough readable.
- Published
- 1991