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Single-use (i.e. disposable) bioreactors are commonly applied in biotechnology, as well as in biopharmaceutical industry, to efficient scaling-up of cultures of animal cells. Wave-type agitated systems provide sufficient mass transfer conditions in single-use culture bags. Spherical microcarriers made with biocompatible polymers are applying to obtain higher levels of biomass yield in various types of in vitro systems for culture of anchorage-dependent (i.e. adherent) cells. The aim of the study was to determine the applicability of three spherical microcarriers, i.e. Cytodex 1, Cytodex 3 and Cytopore, which differ in surface properties of granules, for submerged batch culture of mice anchorage-dependent L929 fibroblasts under conditions of wave-type agitation. Based on the results of cultures performed in small-scale wave-agitated system (5 cm3, laboratory cradle), the Cytodex 3 microcarrier has been identified as the spherical granules promoting proliferation of L929 cells. Cytodex 3 has been also successfully applied in the ReadyToProcess WAVETM25 bioreactor system (WAVE 25, GE Healthcare Bio-Sciences AB, Sweden) equipped with polymer-based culture bag in volume of 300 cm3, what resulted in enhanced growth of L929 cells characterized by Y*X/S equaled to 1.48·109 cells·gglucose-1. [ABSTRACT FROM AUTHOR]