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Mycological analyses of infected mountain pine needles were conducted for years 2011−2012. Yellow spots on needles were found in subalpine zone of the Karkonosze National Park. The isolation of fungi was conducted at the end of July in both years when the intensity of disease symptoms on needles was the highest. Infected needles were sampled from Kopa, Śląskie Kamienie, Kocioł Smogorni, Sokolnik, Łabski Szczyt, Szrenica, Kocioł Małego i Wielkiego Stawu study sites. The dominant species isolated from the infected needles was a probably new species of the genus Lophodermium. Except for this pathogen, Lophodermium pinastri, Botrytis cinerea and Epicoccum niger were identified together with several saprotrophic species, e.g. Aspergillus niger., Sylwan 157 (10): 761-768