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Bagmut I. Yu., Zharova N. V., Popova T. M., Zhukov V. I., Stetsenko S. O. Express experimental evalution of mutagenic activity of complex phoshorus-containing organic mixtures = Експрес-експериментальна оцінка мутагенної активності складних фосфоровмісних органічних сумішей = Экспресс-экспериментальная оценка мутагенной активности сложных фосфоросодержащих органических смесей Journal of Education, Health and Sport. 2016;6(10):443-454. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.164190 http://ojs.ukw.edu.pl/index.php/johs/article/view/3971 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015). 755 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author (s) 2016; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 02.09.2016. Revised 24.09.2016. Accepted: 30.10.2016. UDK 579.695 EXPRESS EXPERIMENTAL EVALUATION OF MUTAGENIC ACTIVITY OF COMPLEX PHOSPHORUS-CONTAINING ORGANIC MIXTURES I. Yu. Bagmut, N. V. Zharova, T. M. Popova, V. I. Zhukov, *S. O. Stetsenko Kharkiv Medical Academy of Postgraduate Education; *Kharkiv National Medical University Summary Researching the mutagenic activity of organic phosphorus-containing mixtures of Esherichia Coli bacteria strain trp A 223 and the continuous cells culture of murine myeloma X63 also detected the presence of weak mutagenic properties in this group of xenobiotics. The concentrations of all compounds 400 and 1000 mg/l have bacteriostatic effect, and their lower concentrations in the range from 10 to 80 mg/l stimulate the mutagenic activity. Phosphorus-containing organic compounds can inhibit the synthesis of proteins, RNA and DNA in cell culture Vero depending on the concentration. Key words: xenobiotics, carcinogenesis, mutagenesis, atherogenesis, suppression of immune deficiency, reproductive function, Esherichia Coli, cells culture of murine myeloma X63. ЕКСПРЕС-ЕКСПЕРИМЕНТАЛЬНА ОЦІНКА МУТАГЕННОЇ АКТИВНОСТІ СКЛАДНИХ ФОСФОРОВМІСНИХ ОРГАНІЧНИХ СУМІШЕЙ І. Ю. Багмут, Н. В. Жарова, Т. М. Попова, В. І. Жуков, * С. О. Стеценко Харківська медична академія післядипломної освіти; *Харківський національний медичний університет, м. Харків Резюме Дослідження мутагенної активності фосфорвмісних органічних сумішей на бактеріях Esherichia Coli штам trp A 223 і перевиваємій культурі клітин мишачої мієломи Х63, виявили наявність слабких мутагенних властивостей досліджуваних ксенобіотиків. Всі сполуки в концентраціях 400 і 1000 мг/л володіють бактеріостатичним ефектом, а в більш низьких концентраціях в діапазоні від 10 до 80 мг/л вони стимулюють мутагенну активність. В залежності від концентрації, фосфорвмісні органічні сполуки здатні пригнічувати синтез білків, РНК і ДНК в культурі клітин Vero. Ключові слова: ксенобіотики, канцерогенез, мутагенез, атерогенез, пригнічення імунологічної недостатності, репродуктивна функція, бактерії Esherichia Coli, клітини мишачої мієломи Х63. ЭКСПРЕСС-ЭКСПЕРИМЕНТАЛЬНАЯ ОЦЕНКА МУТАГЕННОЙ АКТИВНОСТИ СЛОЖНЫХ ФОСФОРОСОДЕРЖАЩИХ ОРГАНІЧЕСКИХ СМЕСЕЙ И. Ю. Багмут, Н. В. Жарова, Т. М. Попова, В. И. Жуков, *С. О. Стеценко Харковская медицинская академия последипломного образования; *Харковский национальный медицинский университет, г. Харьков Резюме . Исследования мутагенной активности фосфосодержащих органических смесей на бактериях Esherichia Coli штамм trp A 223 и перевиваемой культуре клеток мышиной миеломы Х63 выявили наличие слабых мутагенных качеств исследованных ксенобиотиков. Все соединения в концентрациях 400 і 1000 мг/л обладают бактериостатическим эффектом, а в больших концентрациях в диапазоне от 10 до 80 мг/л они стимулируют мутагенную активность. В зависимости от концентрации, форсфорсодержащие органические соединения способны угнетать синтез белков, РНК и ДНК в культуре клеток Vero. Ключевые слова: ксенобиотики, канцерогенез, мутагенез, атерогенез, угнетение иммунологической недостаточности, репродуктивная функция, бактерии Esherichia Coli, клетки мышиной миеломы Х63.

Atherosclerosis together with its cardiovascular consequences is the most common and significant cause of death, particularly in highly developed countries. The process of atherogenesis begins as soon as in childhood and depends on classical risk factors. Atherosclerosis also results from a chronic inflammatory-immune process which takes place in the vascular walls. Furthermore, it has been known for a number of years that the development of atherosclerotic lesions is closely connected with the concentration of homocysteine in serum. Homocysteine is a sulfur amino acid originating from methionine. An increased concentration of homocysteine in blood harmfully influences blood vessels, leading to a higher risk of ischemic heart disease and stroke. Since tackling classical atherosclerosis risk factors is not efficient enough when it comes to protecting the cardiovascular system from diseases, new substances possessing anti-atherogenic properties, especially endogenous ones, are sought. Recently, researchers have paid attention to a connection between homocysteine and an endogenous tryptophan derivative, kynurenic acid. Recently, it was revealed that kynurenic acid counteracts the harmful effects of homocysteine on endothelium cells in vitro. The hypothesis assuming homocysteine-kynurenate interplay suggests the existence of a new mechanism of atherogenesis and gives us an opportunity to use this knowledge in both prevention and treatment of cardiovascular diseases. [ABSTRACT FROM AUTHOR]

Atherogenesis is the process of atherosclerotic plaque formation, leading to coronary artery heart disease. This process involves immune cells, mainly T and B cells, monocytes and macrophages. The process of atherogenesis is induced by inflammatory damage of endothelial cells. The characteristic construction features of the atherosclerotic plaque is a predisposing factor for acute coronary syndromes. The accumulation of inflammatory cells in the artery inner membrane enhances the local inflammatory process due to the secretion of reactive oxygen species, inflammatory cytokines and metalloproteinases, which accelerate the development of atherosclerotic lesions in the arteries. In chronic inflammation of endothelial cells, which is atherosclerosis, there is a decrease in the concentration of elastin and collagen as a result of the increased apoptosis of smooth muscle cells of the intima. This reduces the integrity and strength of the fibrous cap that covers a layer of thrombogenic plaque from contact with blood elements. Permanent inflammation promotes the formation of necrotic core, composed of dead smooth muscle cells, macrophages and foam cells formed by phagocytosis of oxidized lipid molecules. The thin fibrous cap and a large necrotic core are the cause of plaque rupture and thrombus formation within the coronary artery.