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Background: Selenoprotein P (Sepp1) is the major selenoprotein in plasma and contains over 50% of the total plasma. The direct and indirect effects of Sepp1 on anti-oxidant defense can be responsible for its role in cancer risk. One of the common polymorphism of SEPP1gene is the Ala234Thr single nucleotide polymorphism (SNP) in coding region. In this research we studied the association between SEPP1 Ala234Thr (rs3877899) polymorphism with the risk of breast cancer. Materials and Methods: In this case-control study, 162 patients with breast cancer were compared with 227 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes. Determination of DNA genotyping in SEPP1 Ala234Thr (rs3877899) polymorphism was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) by using MwoI enzyme. Statistical analysis was performed by using the MedCalc program and χ2-test. Results: The genotype frequencies were significantly different between the cases and controls. AA homozygosity subjects were more at risk for breast cancer than others (OR=3.23; 95%CI, 1.79-5.84; p=0.001). Conclusion:Based on the results of this study, The Ala234Thr polymorphism of the SEPP1 gene may be associated with the potential for breast cancer in the female population of northern Iranian women. However, studiesneed to be conducted in larger populations to confirm these results.

Background: Selenoprotein P (Sepp1) is the major selenoprotein in plasma and contains over 50% of the total plasma. The direct and indirect effects of Sepp1 on anti-oxidant defense can be responsible for its role in cancer risk. One of the common polymorphism of SEPP1gene is the Ala234Thr single nucleotide polymorphism (SNP) in coding region. In this research we studied the association between SEPP1 Ala234Thr (rs3877899) polymorphism with the risk of breast cancer. Materials and Methods: In this case-control study, 162 patients with breast cancer were compared with 227 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes. Determination of DNA genotyping in SEPP1 Ala234Thr (rs3877899) polymorphism was performed by the polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) by using MwoI enzyme. Statistical analysis was performed by using the MedCalc program and χ2-test. Results: The genotype frequencies were significantly different between the cases and controls. AA homozygosity subjects were more at risk for breast cancer than others (OR=3.23; 95%CI, 1.79-5.84; p=0.001). Conclusion:Based on the results of this study, The Ala234Thr polymorphism of the SEPP1 gene may be associated with the potential for breast cancer in the female population of northern Iranian women. However, studiesneed to be conducted in larger populations to confirm these results. [ABSTRACT FROM AUTHOR]

Introduction: Selenium is an essential element for human health and it is toxic at high concentrations. Selenium is a constituent component of selenoproteins that have enzymatic and structural roles in human biochemistry. Selenium is a best antioxidant and catalyst for production of thyroid hormone. This element has the key role in the immune function; prevention of AIDS progression and the deactivity of toxins. Furthermore, selenium is essential for sperm motility and can reduce abortions. Selenium deficiency was also associated with adverse mood states. The findings regarding cardiovascular disease risk related to selenium deficiency is unclear, though other conditions such as vascular inflammation, oxidative stress and selenium deficiency can cause this disease too. Moreover, consuming of 60 mg of selenium per day may be associated with reduction of cancer risk. In this study, a review of studies has been performed on the biochemical function of selenium toxicity, and its effects on human health. Furthermore, certain identified cancers associated with selenium have been discussed to absorb more attention to the status of this element and also as a guide for further studies. Selenium plays the dual character (useful and harmful) in human health, and then it is necessary to determine the concentration of this element in body fluids and tissues. An appropriate method for routine measurement of selenium in clinical laboratories is electro thermal atomic absorption spectrometry (ETAAS) with very low detection limit and good precision.