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Background & Objective: Successful root canal therapy requires knowledge of the anatomy of the root canal system and complete clearance. Given the possibility of a second canal in mandibular incisors, it is important to find and completely debride this canal Therefore, in this study, we investigated the anatomy of the root canal of mandibular incisor teeth using CBCT.Material & Methods: In this descriptive analytical and cross-sectional study, CBCT images were evaluated from three sagittal dimensions (number of roots, channel type, apical foramen deviation from radiographic apex), coronal (apical foramen deviation from radiographic apex) and axial (channel type). Data were analyzed in SPSS software (Version 2016) through descriptive statistics using frequency distribution and Chi-square test. cases less than 0.05 were considered significant. Results: In this study, 606 mandibular incisor teeth were examined. The mean deviation of apical foramen from radiographic apex was 0.431 ± 0.296 mm. Only 1 double-rooted tooth was observed. 34.5% of the teeth were double-channel and 65.5% were single-channel. Class 1 and 3 Vertucci morphology was the most common and other classes included about 1.5% of the samples.Conclusion: According to the results, mandibular incisors in females and males are more single rooted and have a single canal. Regarding the Vertucci classification, the most prevalent type was type I.