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Background and Objectives: AIDS is a public health problem for which screening tests are recommended for prevention. This study aimed to determine the impact of an educational intervention based on the precaution adoption process model on HIV screening. Materials and Methods: A semi-experimental study was conducted on 120 third and fourth-year nursing students in Sabzevar city in 2019 using random sampling. Data collection tools, researcher-made questioner with appropriate validity and reliability, included demographic information, awareness, and constructs of the precaution adoption process model. Data was collected before, two weeks, and two months after the intervention in intervention and control groups. Data was analyzed using SPSS19 software and tests including Chi-square, independent t-test, GEE, and multiple linear regression. Results: In the present study, 21.7%, 35%, 28.3%, and 15% of students were in stages 2, 3, 4, and 5, respectively. After the intervention, 55% of individuals in the intervention group progressed to stage 6 (action). The educational intervention increased awareness by 4%, sensitivity by 6%, perceived benefits by 16%, and self-efficacy. Additionally, the behavior of the intervention group improved more than three times compared to the control group (P=0.028). Conclusion: The study results indicated that the educational intervention to students, despite being implemented at various stages of the model, increased the likelihood of them moving closer to the stage of performing the behavior. Therefore, designing educational interventions to promote the target group from stages of non-engagement or decision-making to HIV testing is recommended. [ABSTRACT FROM AUTHOR]