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Background and Objectives: One of the most important factors in pathogenic disease is the attachment of pathogenic bacteria to the host cell surface. The present study was conducted to introduce an alternative bio-drug that prevents the attachment of microorganisms and the onset of disease. Subjects and Methods: This study was carried out during the three experimental periods. Clinical and standard strains of E. coli and C. albicans yeast were collected from the Khalat-Pushan Research Station of Tabriz University and Iran Research Organization respectively. Co-aggregation activity of treatments with both E. coli and C. albicans were investigated by Collado method and expressed as percentage. Anti-adhesive activity was also determined by microtitre plate method and calculated by reduction in attachments percentage. Results: The highest co-aggregation effect with 74.68% was related to “probiotic+ nano-selenium” treatment with standard strain of C. albicans and “nano-selenium”. While treatment with 39.32% had the lowest co-aggregation activity with clinical strain of E. coli (P<0.05). In determination of anti-adhesive activity of experimental treatments, except for the “probiotic” treatment on clinical strain of C. albicans, there was no significant difference among treatments for both species of E. coli and C. albicans. In both strains of E.coli and C. albicans, the standard strains showed significantly higher sensitivity. Conclusion: All the experimental treatments had a good coaggregation and anti-adhesive ability with pathogenic species. “Probiotic + nano-selenium” treatment had relatively high ability during three experimental protocols Therefore, it can be used as an appropriate antibiotic alternative for the prevention or control of disease. [ABSTRACT FROM AUTHOR]