5 results on '"Alizadeh, H."'
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2. Several fish species carp culture with Chinese black carp to increase diversity in warm water fish farms
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Sadeghinejad Masuoleh, Esmaeil, Mehdinezhad, K., Fallahi, M., Morhdi, M., Zibsayadan, M., Khoshal, J., Sayad Borani, M., Sharifian, M., Reza Alizadeh, H., Daghigh Rohi, J., Abedini, A., Mehdizadeh, G.R., Darvishi, S., Ghnaatprast, A., Dansh, A., Mazdy, J., Ghasemi, M., Hossynjani, A., Saberi, H., Babaei, H., Amiri, A., Sabk Ara, J., Khatib, S., Yousefzad, E., Moradi, M., and Nasiri, T.
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Species ,Diversity ,Survival rate ,ANOVA ,Silver Carp ,Warm water ,Carp culture ,Growth ,Bighead carp ,Common carp ,Grass carp ,Fish ,Chinese black carp ,sense organs - Abstract
The objective of this study was to investigate the effects of black carp introduction in chines carp poly culture as well as its effect on the growth, survival rate and production of chines carp species. This study conducted in two consecutive year. The period of culture in first year was during Jun 1 until Des 5 ,2009 and in second year it was from Jun 4 to Dec, 1,2010 .The experiment were started by selecting 9 earth end pond each with 800 m2 area and was performed in triplicate with 2 treatment and one control , each year .The ponds were treated by liming and fertilizer before filling water and then stocked with 3000 chines carp ( 140 Silver carp , 42 Grass carp , 42 Common carp and 25 Bighead carp ) while the number of Black carp in treatments 1, 2, 3 and 4, respectively, 250, 500, 75 and 125 pieces in Ha, blackcap was added to the control species respectively . The number of fish species harvested at end of culture in treatment 1, 2 and control .The total fish species harvested at the end of first year was 2276 out 1904 species released. The number of fishes catch in treatment 1,2 and control were 637,658and 573 species. the statistical analysis showed that average weight of black carp were 407±150 gr in treatment 1, 234±121gr in treatment 2 with the minimum and maximum weight of 242 and 780 gr in first treatment as well as 110 and 750 gr in second treatment the production of black carp in first treatment were 42 kilogram per hectare and 60 kilogram in second treatment. The growth rate were higher in second treatment than first one. In second year the investigation repeated in triplicate with treatment3, 4 and one control .The total stocking density in treatment 3,4 and control were 665,633, and 574 pieces. Statistical analysis showed that total weight of black carp in third treatment was2049 ±614gr and in forth treatment was 2361 ±60 gr where the minimum and maximum values were 3115 gr and 1025 gr respectively. The survival rat of black carp in treatment3 higher than in were treatment 4 while the growth in treatment 4 was higher treatment3.The total production of black carp in treatment 3 and 4 were 123 and 181kilogram per hectare respectively. The ANOVA test conducted for grass carp revealed that treatment 4 is significantly difference with treatment 3 and control. While for silver carp the treatment 4 is significantly difference with treatment 1 and control while for Big head carp the treatment 4 was significantly difference with control at 5% level (P< %5) .On second year the black carp reached to acceptable market size which could be compatible with other carp such as common carp and grass carp comparative analysis between different stocking density indicate that the stocking density with 125 pieces showed the highest positive effects. Iranian Fisheries Science Research Institute Published
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- 2013
3. Evaluation of Antibacterial Effects of Lecanora muralis SP. Extract on Staphylococcus Aureus and Salmonella Typhimurium in in-vitro and in animal model.
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Semnani, Sh Nasisri, Rahnema, M., Ghasempour, H., and Alizadeh, H.
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LECANORACEAE ,STAPHYLOCOCCUS aureus ,SALMONELLA typhimurium ,PLANT extracts ,ANTIBACTERIAL agents - Abstract
Background & Objective: Staphylococcus aureus and Salmonella typhimurium are among important causes of infection and antibiotic resistance. It is essential to find antimicrobial compounds against them. The objective of this study is to evaluate the antibacterial activity of Lecanora muralis SP. extracts on Staphylococcus aureus and Salmonella typhimurium in in-vitro and in animal model. Materials & Methods: In this study, aquatic, acetonic, and ethanoic extracts of Lecanora muralis SP. were prepared. Then their antimicrobial effects were determined by agar well diffusion method. Minimum Inhibitory Concentration (MIC) and Minimum Bacterial Concentration (MBC) were calculated by macro dilution method. In animal model, 24 hours after inoculation of bacteria in mice, care was provided by injecting 0.5 ml of Lecanora muralis SP. extracts (as MBC concentration).. Then, the colonies of mentioned bacteria in spleen were counted on agar media after nine days. Results: The results indicated that MIC of acetonic extract of Lecanora muralis SP. for Staphylococcus aureus and Salmonella typhimurium were 103.75 and 207.5 mg/ml and for ethanoic extract were 49.81 and 99.625 mg/ml, respectively. The aquatic extracts of the tested lichens didn't show any antibacterial activity on mentioned organisms. The mean of the bacterial count in experiment group showed significant reduction compared to the control group in animal model. Conclusion: The ethanoic extract of Lecanora muralis SP. had the most effective antimicrobial activity on Staphylococcus aureus and Salmonella typhimurium in comparison with acetonic extract. In addition Salmonella typhimurium was more sensitive to extracts. [ABSTRACT FROM AUTHOR]
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- 2014
4. Antibacterial Effect of Allicin, Silver Nano Particles, and Their Combination against Skin Infection due to Pseudomonas aeruginosa in Animal Model.
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Mirzaei, F., Salouti, M., Shapouri, R., and Alizadeh, H.
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ANTIBACTERIAL agents ,PSEUDOMONAS aeruginosa infections ,SKIN disease treatment ,ORGANOSULFUR compounds ,SILVER nanoparticles - Abstract
Background & Objective: Common antibiotics may be useless against multiple pathogens because of the multidrug resistance. Therefore, new ways of nanotechnology and effective herbal combination can be the solution for this problem. The objective of this study is to investigate the antimicrobial effect of Allicin, silver nano particles and their combination against skin infection due to Pseudomonas aeruginosa in animal model. Materials & Methods: In this experimental study, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Allicin, silver nano particles, and their combination were determined by macro dilution susceptibility test. Skin infection was produced in the shoulder region of 12 mice using Pseudomonas aeruginosa and the effect of ointment of Allicin, silver nano particles, and their combination were evaluated. Results: The results showed that MIC and MBC of Allicin for pseudomonas aeruginosa were 2.38 and 4.77 mg/ml, respectively. MIC and MBC of silver nano particle for Pseudomonas aeruginosa were 3.12 and 6.25 ppm, respectively. MIC and MBC of combination of Allicin and silver nano particles on Pseudomonas aeruginosa were 0.59 mg/ml and 1.5 ppm, 1.19 mg/ml and 3.12 ppm, respectively. Anti-microbial effect of Allicin, silver nano particles and the synergistic activity of their combination against skin infections due to Pseudomonas aeruginosa was observed in animal model. Conclusion: The results showed that Allicin in combination with silver nano particles have synergistic effect against skin infection due to Pseudomonas aeruginosa. [ABSTRACT FROM AUTHOR]
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- 2014
5. Effect of a protrombin activator isolated from Iranian Echis carinatus venom on hemostasis.
- Author
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Babaie, M., Zolfagharian, H., Salmanizadeh, H., Zare Mirakabadi, A., and Alizadeh, H.
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VENOM ,BLOOD coagulation ,PROTHROMBIN ,METALLOPROTEINASES ,BLOOD vessels - Abstract
Background and Objectives Echis carinatus venom is a complex mixture of toxins. This venom contains metalloproteinases which convert prothrombin to meizothrombin. The prothrombin activator leads to the formation of small blood clots inside the blood vessels throughout the body. To understand the effect mechanism of Iranian Echis carinatus venom, in this study we investigated the effect of EV on human plasma proteins (prothrombin and fibrinogen) and on blood coagulation. The aim was the purification and characterization of procoagulant factor from the Iranian Echis Carinatus venom and the evaluation of the procoagulant activity on human plasma. Materials and Methods Crude venom from the Iranian snake species E. carinatus was selected. The prothrombin activator was purified from the crude venom of Echis carinatus by combination of the procedures by gel filtration, ion-exchange chromatography, and reverse phase HPLC. Electrophoresis on 12.5% polyacrylamide gel was performed. Results The Iranian E. carinatus venom was able to coagulate human plasma very rapidly. The coagulation time was reduced from 13.4 seconds (SD ±0.59) to 8.6 seconds (SD= ±0.64) when human plasma was treated with crude venom (concentraion of venom was 1 mg/ml). Conclusions The venom of Iranian Echis carinatus contains procoagulant factors. It seems the fraction F1B4 isolated from lEc to be like coagulation proteins which coagulate human plasma very rap idly in vitro. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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