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Background and purpose: Toxoplasmosis is a parasitic protozoan disease producing severe complications in congenital and immunocompromised cases. The enzyme-linked immunosorbent assay (ELISA) is the most common laboratory method for serodiagnosis of toxoplasmosis. Commercial kits have no unique procedure for standardization and this may influence the consistency of the results. The present study was designed to determine the reproducibility of results obtained by ELISA in different laboratories using similar commercial kits. Also, the results obtained from different commercial kits were compared. Materials and methods: Three Toxoplasma gondii IgG-ELISA kits from different commercial companies were used and the results reported by three different university laboratories evaluated. Eighty one serum samples from patients referred to a private laboratory for serologic determination of toxoplasmosis were examined. The results of the tests were reported as qualitative values (positive, negative, borderline), and the agreement rates determined using the Kappa coefficient. Results: Comparing three different kits, the results of 76 serum samples were similar and the Kappa coefficients calculated at 0.85, 0.90, and 0.97. When comparing different laboratories, the results of 80 serum samples among three laboratories were similar and the Kappa coefficients calculated at 0.97-1. Conclusion: Based on the results of the present study, it could be concluded that the ELISA test for detection of antibodies to T. gondii has the potential to produce consistent results in different laboratories while using different kits. [ABSTRACT FROM AUTHOR]