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The purpose of this study was to predict the impact of managers' ethical and spiritual behavior on the establishing transformational leadership at the University of Isfahan. Research method was correlational-descriptive and statistical population included all employees of the University of Isfahan at 1392-93 academic year (1230). Sample size was calculated from krejcie and Morgan table (201), and 188 questionnaires were returned. Because the number of employees was different in the departments and faculties, a stratified random sampling method was used. Measurement tools were a researcher-made questionnaire based on Reave (2005) and Fry (2003) studies with 16 items, and Bass (1985) transformational leadership questionnaire. Data analysis were performed based on correlation- coefficient and regression analysis. Findings showed that there is a significant and positive correlation between ethical and spiritual behaviors of university managers with transformational leadership ( 0.05). In addition, the components of the spiritual leadership (development of human culture, Job vision, integrity, honesty and humility) significantly predict the four components of transformational leadership style (idealized influence, inspirational motivation, stimulation intellectual and individual considerations). [ABSTRACT FROM AUTHOR]