Search

Background and aims: Phenylhydrazine (PHZ) as a well-known hemolytic agent can induce a variety of adverse effects including hepatotoxicity. The main goal of the present study was to examine the possible efficacy of vitamin C against PHZ-induced oxidative damages and apoptosis in mouse liver. Methods: In this randomized controlled experimental study, adult male mice were assigned to four groups of eight mice each. Two groups of mice received PHZ at a dose of 60 mg/kg per 48 hours intraperitoneally for 35 days. Vitamin C was administered to one of these groups at a dose of 250 mg/kg per day intraperitoneally four hours before PHZ injection. A vehicle-treated control group and a vitamin C-only treated group were also included. Serum and hepatic samples were collected 24 hours after the last treatment and subjected to biochemical and histomorphometric and histochemical examinations, respectively. The data were analyzed by one-way analysis of variance followed by Duncan test for post hoc comparisons. Results: PHZ treatment caused a significant elevation (P < 0.05) in serum concentrations of aspartate transaminase, alanine transaminase, alkaline phosphatase, lactate dehydrogenase and malondealdehyde and decreased serum level of total antioxidant capacity, superoxide dismutase and albumin. Moreover, PHZ significantly increased apoptosis and diameters of hepatocytes and their nuclei as well as number of Kupffer cells. Remarkably, vitamin C pretreatment improved all changes in the above-mentioned parameters. Conclusion: Vitamin C can modulate PHZ-induced oxidative injuries and apoptosis in mouse liver due to its antioxidant activities. [ABSTRACT FROM AUTHOR]

Background and aims: The reduction in blood flow may result pathological changes in testicular and spermatogenesis function. Moreover, free iron ions result of lysing red blood cells and consequently the increase in iron ions content of testes leads to oxidative stress with reproductive system dysfunction. So, the present study was aimed to evaluate the protective effect of the simultaneous use of royal jelly (RJ) and vitamin C (Vit C) on hemolytic anemia condition along with decomposition of red blood cells. Methods: In this experimental study, 32 mature mice weighting 20-25 gr were divide into 4 groups including control group received normal saline (0.2 mL, IP) with intraperitoneal injection, sham control group administered with Phenylhydrazine (PHZ) (6 mg/100g , IP), PHZ+ Vit C with (250 mg/kg, IP)+ RJ (100 mg/kg,) group orally and RJ+Vit C group. After 35 days, blood samples were obtained from heart with the evaluation of serum levels of total antioxidant capacity (TAC) and malondialdehyde (MDA). Also, the sperm quality including sperm motility, viability, sperm count, chromatin condensation and DNA damage was assessed. Results: There was a significant reduction in TAC level of PHZ group in the present study, and co-administrating Vit C and RJ in receiver group of antioxidants could prevent this decrease. Moreover, the PHZ caused the serum level of MDA is increased. Meanwhile, co-administrating the Vit C and RJ diminished MDA in PHZ-administrated animals. Finally, co-administrating the Vit C and RJ by scavengering of free radicals resulted in remarkable enhancement in quality of sperm parameters (P<0.05). Conclusion: PHZ can disorder sperm parameters through inducing oxidative stress. Co-administrating of the Vit C and RJ may be significantly up-regulated the serum TAC level and down regulating of MDA promoted the sperm quality. [ABSTRACT FROM AUTHOR]

Background and aims: Hemolytic anemia-induced hypoxia can lead to multi-organ dysfunctions. The aim of the present study was to explore the efficacy of vitamin C as an antioxidant agent against hemolytic anemia-induced changes in small intestine histoarchitecture of phenylhydrazine (PHZ)-treated mice. Methods: In this experimental study, adult male mice were randomly assigned to four groups of eight mice each. PHZ was administered to two groups of mice at a dose of 60 mg/kg per 48 hours intraperitoneally for 35 days. One of these groups received vitamin C (250 mg/kg per day) intraperitoneally four hours before PHZ administration. A vehicle-treated control group and a vitamin C control group were also included. 24 hours after the last treatment, desired segments of small intestines were dissected out and subjected to histological processing and morphometric parameters were evaluated. Results: PHZ caused significant decreases in villi width of duodenum and jejunum, crypts depth of duodenum, distribution rate of the goblet cells in ileal villi and height of villi in all segments of small intestine. Vitamin C markedly improved all changes in the aforementioned parameters. Conclusion: Vitamin C could ameliorate hemolytic anemia-induced histological injuries in mouse small intestine. [ABSTRACT FROM AUTHOR]