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Background: Aromatase is a key enzyme in production of estrogen. This enzyme is encoded by cytochrome P-450 (CYP19) gene. It has been shown that overexposure of cells to estrogen causes breast carcinoma and breast cancer patients show higher level of aromatase expression. The purpose of this study was to investigate polymorphism of the CYP19 intron 4 (TTTA)n among breast cancer patients and healthy individuals and its relation to risk of breast cancer. Methods: This study was a case-control study on 100 patients and 100 controls women. DNA was extracted from the blood of study subjects, the sequence were amplified by the technique of Polymerase Chain Reaction (PCR). Thereafter the number and sequence of TTTA was measured by gel electrophoresis on Polyacrylamid. Findings: The results of this study shown that CYP19 gene allele distribution in Isfahan population varies between 7 and 13. The most common allele in both controls and cases was (TTTA)7-TCT with frequencies of 34.3% and 34.8%, respectively. Conclusion: Our results show that the frequency distribution of 10 TTTA repeats is more in controls (2.3%) than in patients (1.3%). This discrepancy suggests a protective role for this allele for Isfahanian women. [ABSTRACT FROM AUTHOR]