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Nowadays, with the increase in diseases and consumers’ preference for organic foods, the use of natural plant compounds to improve and increase their shelf life is on the rise. In addition, the use of medicinal plants, which can have therapeutic properties, has attracted attention. Black seed oil has attracted a lot of attention in the past due to its unique therapeutic properties. The aim of this study was to investigate the antioxidant activity and evaluate the antimicrobial activity of black seed oil against Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus. The amount of phenolic compounds in black seed oil was measured by Folin-Ciocalteu. The antioxidant activity was determined by DPPH and ABTS radical scavenging methods. The antimicrobial effect of Nigella sativa oil was investigated according to well diffusion agar, disk diffusion agar, interaction, minimum inhibitory concentration and minimum bactericidal concentration methods.The Nigella sativa oil had 16.25 mg GAE/g phenolic compounds. The antioxidant activity based on DPPH and ABTS radical scavenging were 86.62% and 94.85%, respectively.The Nigella sativa oil showed a significant antimicrobial effect on the tested microorganisms. In the combined mode (interaction) of Nigella sativa oil with chloramphenicol antibiotic, synergistic mode was observed for all bacterial strains. Given the significant antioxidant activity and antimicrobial effect of Nigella sativa, it can be used in food and pharmaceutical industries. [ABSTRACT FROM AUTHOR]

Chemical properties and antimicrobial effect of Thymus trautvetteri essential oil against some of the bacteria causing infection and food poisoning were evaluated in this study. For this purpose, T. trautvetteri essential oil was extracted with the help of hydrodistillation method and its total phenol content was based on Folin-Ciocalteu method, its antioxidant activity was based on DPPH and ABTS free radical inhibition methods and its antibacterial effect against Escherichia coli, Shigella dysenteriae, Staphylococcus aureus and Bacillus cereus were investigated based on disc diffusion agar, well diffusion agar, minimum inhibitory concentration and minimum bactericidal concentration methods. The results showed that T. trautvetteri essential oil contained 6.27 mg of catechin/g total phenol and its inhibitory activity against DPPH and ABTS free radicals was obtained as 12.32 and 10.20 mg/ml, respectively. The results of the antibacterial activity of the essential oil showed that S. aureus and E. coli were the most sensitive and resistant bacterial strains against T. trautvetteri essential oil, respectively. The inhibition zone in the disc diffusion agar and well diffusion agar methods, and the minimum inhibitory and bactericidal concentrations for S. aureus were 14.60 mm, 16 mm, 1 mg/ml and 4 mg/ml, respectively. According to the findings of this research, T. trautvetteri essential oil can be used as a natural antioxidant and antimicrobial agent to prevent oxidative reactions and microbial spoilage in food. [ABSTRACT FROM AUTHOR]