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In this study, the effect of amplitude (25-75%), sonication time (50-100min) and ethanol concentration (30-70%) at ambient temperature on the crude extraction yield, total phenolic and total flavonoid contents of garlic was studied and optimized using response surface methodology due to the importance of using emerging techniques in bioactive compounds extraction process from plant sources. The result of numerical optimization showed that the highest amount of crude extraction yield of 27.34%, total phenolic content of 27.59 (mg GAE/g of extract) and total flavonoid content of 5.09 (mg CAE/g of extract) are achievable using ultrasound intensity of 57%, ultrasound duration of 100 min and 70% ethanol concentration. The DPPH free radicals scavenging activity and FRAP for the extract obtained under optimal conditions were 77.57% and 3.21 mM FeSO4/L. The quantitative and qualitative properties of the extract obtained under ultrasound-assisted extraction optimized conditions were significantly (p<0.05) higher than those of control (absence of ultrasound) indicating that the ultrasound technique is desirable for bioactive compounds extraction. [ABSTRACT FROM AUTHOR]