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Introduction Hyperbilirubinemia is the most common cause of neonatal hospitalization in the first month after birth. Understanding the underlying causes of jaundice helps identify newborn at risk faster. The aim of this study was to determine the prevalence of jaundice in neonates with diabetic mothers on the 3rd to 5th day of after birth. Material and Method 120 pregnant mothers aged 18 to 40 years who had diabetes during pregnancy (familial or gestational diabetes) and were treated with insulin were included in this descriptive epidemiological study. All demographic information of the mother and infant is recorded in the checklists and after delivery, mothers are advised to visit the clinic on a routine checkup for jaundice (jaundice visible in the sclera) on the third to fifth day after birth. After a thorough neonatal examination, a total bilirubin test will be performed. Results Of 120 diabetic mothers, 54 (45%) had no jaundice, 24 (20%) had clinical jaundice, and 42 (35%) needed phototherapy. Statistical analysis showed that there were no significant differences between the three groups without jaundice, clinical jaundice and need for phototherapy in terms of gender, gestational age and Rh factor incompatibility but there was a significant difference between type of feeding, birth weight and incompatibility of ABO system. . Further statistical analysis showed that only birth weight factors and blood group incompatibility were associated with icterus. Conclusion Maternal diabetes and neonatal jaundice appear to be associated. [ABSTRACT FROM AUTHOR]