1. Métodos utilizados para la selección de parentales en pre-mejoramiento genético de plantas.
- Author
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Carrodeguas-Gonzalez, Ayerin and Zúñiga-Orozco, Andrés
- Subjects
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CYTOLOGICAL techniques , *FLUORESCENCE in situ hybridization , *SELECTION (Plant breeding) , *POLLEN viability , *IN situ hybridization , *POLLINATION , *MITOSIS , *MEIOSIS - Abstract
A key step in the pre-breeding stage of plant breeding is the selection of cultivars with desirable characteristics for use as parents to develop new crop varieties. With the development of technology, numerous laboratory techniques have emerged as key tools for breeding programs to save time and resources. For these reasons, the aim of this review was to describe techniques based on cytological and cytogenetic studies that are feasible for the selection of parents during the pre-breeding stage. Among the cytological techniques addressed in this review are: pollen viability and quality, stigmatic receptivity to know how long the stigma is receptive, and pollen tube growth to study incompatibility events. Another key step in pre-breeding is to determine the chromosomal number of the parents, which is possible by different techniques of classical cytogenetics (studies during mitosis and meiosis) and molecular cytogenetics (flow cytometry, Fluorescent in situ Hybridization and Genomic in situ Hybridization) that are discussed in this review. [ABSTRACT FROM AUTHOR]
- Published
- 2022