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Knowledge in the curriculum especially the National Preschool Standard Curriculum (NPSC) (Revised 2017) which is coordinated for all kindergartens in Malaysia depends on professional teachers to implement it effectively. This survey aims to determine the effect between professional qualifications and years of experience on preschool curriculum knowledge among private kindergarten teachers. Questionnaires as instrument was administered to 269 private kindergarten teachers from Gombak district, Selangor who were selected randomly. The two-way ANOVA analysis showed that professional qualifications had a significant effect while years of experience required a combination of professional qualifications to have a significant interaction effect on preschool curriculum knowledge, yet inversely. Post Hoc comparison shows that the mean score of preschool curriculum knowledge differs significantly between the group of teachers of Diploma in Early Childhood Education (ECE) / Preschool Education and above with the group of ECE Certificate / Preschool Teaching teachers. The profile plot shows the highest interaction effect for ECE Diploma/Preschool Education teachers and above occurs during their experience of Less than 3 years, then the interaction effect in the following year decreases. In conclusion, higher professional qualifications for private kindergarten teachers have a significant impact on their knowledge of NPSC (Revised 2017), while years of experience is an added value during the early years of teaching for higher qualified teachers. The implication is that follow-up action in an effort to help private kindergarten teachers, especially experienced ones, is needed for them to obtain professional qualifications until the ECE Diploma. [ABSTRACT FROM AUTHOR]