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PURPOSE Regular exercise has been shown to have anti-aging effects on the vascular system by improving endothelial function and diminishing oxidative stress. However, adherence to an exercise regimen can be difficult for some individuals, particularly those with limited mobility. This study investigated the effects of resveratrol as an exercise mimetic for preventing and improving oxidative stress-induced senescence in vascular endothelial cells. METHODS Cultured human umbilical vein endothelial cells were used to investigate the reversal effects of laminar shear stress and the protective effects of resveratrol on oxidative stress-induced premature senescence. Senescence was estimated using SA-β-gal staining and western blot analysis, whereas oxidative stress levels were evaluated using MitoSOX staining. Matrigel tube formation and scratch assays were also performed to assess endothelial angiogenic and migration functions. RESULTS The study showed that laminar shear stress could partly normalize premature endothelial senescence. Resveratrol pretreatment attenuated reactive oxygen species production and prevented senescence in H2O2-induced premature aging. Additionally, resveratrol contributed to maintaining endothelial cells’ angiogenic and migratory functions. CONCLUSIONS This study demonstrated that laminar shear stress can mitigate the effects of oxidative stress-induced premature senescence. Resveratrol may be a potential substitute for exercise to prevent age-related damage to the endothelium by increasing resistance to oxidative stress and improving endothelial function.

PURPOSE Impaired autophagy, a conserved process for the cellular energy homeostasis by degrading damaged cytosolic constituents, is an important contributor to the pathogenesis of atherosclerosis. The purpose of this study was to determine whether regular aerobic exercise can improve obesity-induced dysregulation of autophagy in mice arterial endothelial cells. METHODS High-fat diet-induced obese mice were subjected to the treadmill running for 10 weeks (15 m/min, 1 hour/day, 6 days/week). Endothelial cells were isolated from aorta by perfusing with QIAzol reagent. The mRNA and protein expressions of autophagy-related genes were investigated by qRT-PCR and western-blot respectively. Statistical analysis was performed by one-way ANOVA. RESULTS Autophagy-related gene expressions in both levels of mRNA and protein were significantly decreased in vascular endothelial cells and arterial vessels of obese mice. However, these phenomena have been partly reversed by the regular treatment of treadmill exercise for 10 weeks. Furthermore, eNOS downregulation induced by the obesity was also improved by the regular aerobic exercise, representing enhanced endothelial function. CONCLUSIONS The current study has shown that the obesity-induced autophagy dysregulation and vascular dysfunction can be partly improved by the regular aerobic exercise in mice vascular endothelial cells.

Intravascular papillary endothelial hyperplasia (IPEH) has appeared in the literature under a variety of names, including Masson's tumor, Masson's hemangioma, and Masson's pseudoangiosarcoma. It is a benign lesion of the skin and subcutaneous tissue characterized by reactive proliferation of vascular endothelial cells with papillary formations. The clinical picture is not specific and the lesion resembles malignant angiosarcoma clinically and histopathologically. Therefore, it is often mistaken for angiosarcoma and a group of other benign and malignant vascular lesions. We report on a case of IPEH adherent to peripheral nerve treated with operative excision. [ABSTRACT FROM AUTHOR]