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A simple and reliable analytical method has been developed for the determination of pantothenic acid in food. For the high-protein food, 20 mL of water was added to 2 g of sample, and after homogenization extraction, 1 mL of 15% zinc sulfate solution was added, mixed well, centrifuged, and the supernatant was filtered to make the test solution. For the low-protein food, 20 mL of 1% formic acid solution was added to 2 g of sample, homogenized, extracted, centrifuged, and the supernatant was filtered to make the test solution. The HPLC separation was carried out on a L-column2 ODS column with 0.02 mol/L phosphate solution (pH 3.0) acetonitrile (95 : 5) as the mobile phase, and detected at 200 nm. The LC-MS/MS conditions were L-column2 ODS as the separation column, 5 mmol/L ammonium formate (containing 0.01% formic acid)-methanol (85 : 15) as the mobile phase, and multiple reaction monitoring (MRM) was used for detection. The recoveries of pantothenic acid in milk powder and nutritional food products were more than 88% with high precision. As a result of analyzing commetrcially available foods labeled as containing pantothenic acid, analytical values almost identical to the labeled values were obtained, and a high correlation was observed between the values obtained by HPLC and LC-MS/MS. [ABSTRACT FROM AUTHOR]

A simple and reliable analytical method has been developed for the determination of pantothenic acid in food. For the high-protein food, 20 mL of water was added to 2 g of sample, and after homogenization extraction, 1 mL of 15% zinc sulfate solution was added, mixed well, centrifuged, and the supernatant was filtered to make the test solution. For the low-protein food, 20 mL of 1% formic acid solution was added to 2 g of sample, homogenized, extracted, centrifuged, and the supernatant was filtered to make the test solution. The HPLC separation was carried out on a L-column2 ODS column with 0.02 mol/L phosphate solution (pH 3.0)- acetonitrile (95 : 5) as the mobile phase, and detected at 200 nm. The LC-MS/MS conditions were L-column2 ODS as the separation column, 5 mmol/L ammonium formate (containing 0.01% formic acid)-methanol (85 : 15) as the mobile phase, and multiple reaction monitoring (MRM) was used for detection. The recoveries of pantothenic acid in milk powder and nutritional food products were more than 88% with high precision. As a result of analyzing commetrcially available foods labeled as containing pantothenic acid, analytical values almost identical to the labeled values were obtained, and a high correlation was observed between the values obtained by HPLC and LC-MS/MS.

Similarities among non-prescription pharmaceutical vitamin products generally available in community pharmacies were examined based on their vitamin components, and a chart was constructed to differentiate products to assist in appropriate product choice. In the analysis of the similarities, two hundred and seventy-six data entries on vitamin products were extracted from the database on the package inserts of the pharmaceutical products provided by the Pharmaceuticals and Medical Devices Agency, and they were reviewed for the amounts of vitamins they contained, in which the B vitamins, or vitamin B 1 , B 2 , B 6 , B 12 , and niacin, were considered as well as vitamins C and E. Pantothenic acid and L-Cysteine that are frequently used in combination with those vitamins are also taken into consideration. The data entries were then processed by classical multi-dimensional scaling to evaluate the inter-product similarities. As a result, it was shown that the products categorized as pharmaceutical nutrients and tonics containing vitamins (NTcV) are similar to one another, reflecting the fact that they are less characteristic regarding their vitamin components. As for the products in other categories, they were generally found to be featured for their unique content of vitamin components, and thereby, each category includes products with a wide range of variation. It was also indicated that some products categorized as vitamin B 1 , B 2 , and C products are less distinguishable from those categorized as NTcV. These findings will assist pharmacists to decide on an appropriate product for a customer following consultation.

The mechanochemical reaction of free riboflavin (FR) due to vibratory ball milling was carried out in a stainless steel vessel at room temperature under anaerobic conditions. The ESR of the fractured sample showed a broad single-line spectrum. It is suggested that the solid-state single-electron transfer (SSET) reaction from the surface of the stainless steel vessel to FR proceeded during the vibratory milling, resulting in the formation of the corresponding anion radicals. When the mechanochemical reaction of FR in the presence of calcium pantothenate (PC) was carried out, the radical concentration increased with the increasing PC content. It was shown that the anion radical in the metal complex was stable for a lengthy period of time even in highly humid air.