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PCR-RFLP was used for genotyping of GNB3 C/T in 133 patients with EH and 257 healthy control subjects in the high risk population and in 98 patients with EH and 110 healthy control subjects in general population. Some biochemical tests were performed also. The association between the polymorphism and blood pressure was analyzed by the case-control study. Not association between GNB3 825C/T polymorphism and blood pressure was found in both populations. CT and TT genotypes in women of the high risk population are associated with diastolic blood pressure, serum sodium and calcium. Although GNB3 is not a susceptible gene of hypertension in the northeast Chinese, it still has some effects on regulation of the blood pressure in susceptible women.

A cDNA of 1694 bp was cloned from human liver Marathon cDNA by means of Rapid Amplification of cDNA Ends (RACE). The cDNA has an open reading frame at 43-1530 bp encoding 495 amino acid residues and a 17-residue signal peptide. There are four Igc2 domains in the translated polypeptide, which is highly homologous to the human alpha-1B glycoprotein isolated from human plasma. Upon this, we conclude that this cDNA is the alpha-1B glycoprotein precursor gene which has never been cloned before, and it may be a novel member of immunoglobulin superfamily and may involved in the cell recognition and the regulation of cell behavior.