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OBJECTIVES To compare the affective responses and enjoyments to acute high-intensity training (HIIT) with short or long intervals in young and obese females. METHODS Participants completed two bouts of HIIT which were separated over 48 hours respectively. The exercise protocols were cycling on an ergometer at 90%VO2peak intensity for 4 minutes with 3 minutes recovery(Low-frequency interval, Group L) or 40 seconds cycling with 30 seconds recovery (High-frequency interval, Group H) until 200 KJ mechanical work was completed. At the time of 0%, 25%, 50%, 75% and 100% work was completed, the feeling scale (FS), rating of perceived exertion (RPE) were recorded, and the blood lactic acids (LA) were measured when the 50% and 100% work was completed. The physical activity enjoyment scale (PAES) was recorded 5 minutes after the exercise. RESULTS Before the exercise, there were no significant differences between the group H and L in terms of the HR, RPE, FS and LA. When 25%, 50%, 75% and 100% of work was completed, the HR and the RPE in group H were all lower than the corresponding time point in group L. The LA in group H was lower than the corresponding values in group L when 50% and 100% work was completed. At the time of 75% and 100% work was completed, the FS in group H was higher than group L, although there were no significant differences at the time of 25% and 50% work done between the two groups. By the end of exercise, the PAES score in group H was higher than group L. CONCLUSIONS Increasing the frequency of interval could increase the positive feeling during HIIT and improve the physical activity enjoyment.