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Using HS.GC, We have succeeded in simultaneous determination of Ac, MeOH and MEK in urine without any complicated pretreatment or correction by internal standard. Moreover, in order to lower the detection limits of these materials, study was made on the salting out effect using 14 kinds of salts. As pretreatment, 2.0 ml of urine, 3.0 g of sodium sulfate and small sized magnetic stirrer are put into vial, which is sealed by septum. This is then heated for 10 min in warm bath of 50 degrees C. In order to dissolve the added salts as much as possible, the specimen is stirred by the stirrer. After cooling the liquid to room temperature, the specimen is analysed by HS.GC. The results showed that sodium sulfate was excellent synthetically. 1) Using the urine of workers not exposed to organic solvents three kinds of urine having specific gravity of 1.010, 1.024 and 1.034 were prepared and mixed standard organic solvents (Ac, MeOH and MEK) were added. Recovery percentages and coefficients of variation were calculated. The results showed that recovery percentages ranged from 92.0 to 101.7% and coefficients of variation from 0.2 to 4.6%. 2) The regression equations of standard curves were satisfactory with y = 9053x - 200(r = 0.999, n = 12) for Ac, y = 801x - 400 (r = 0.999, n = 12) for MeOH, and y = 15488x - 277 (r = 0.999, n = 12) for MEK. 3) The detection limits calculated by IUPAC formula were 0.0092 mg/l for Ac, 0.11 mg/l for MeOH and 0.0063 mg/l for MEK. These results indicated that this method is superior to other methods because the pretreatment is very simple, specificity is excellent, analysis by standard curves is possible, and this method is not affected by specific gravity of the urine.

In 9 series of physical examinations legally required for trichloroethylene (TCE) workers, levels of urinary total trichloride compounds (TTC) were revealed to be generally high in 323 specimens, including 250 male ones, collected from a medium sized company. Sixty four specimens (19.8%), including 56 male ones (22.4%), exceeded 300 mg/l which are regarded to correspond to the threshold limit for TCE in the air of the work site. Four specimens had over 900 mg/l. In all 323 specimens, however, no abnormalities were found with respect to specific gravity of blood, hemoglobin concentration and urinary tests. TTC concentration values increased in specimens in the last 4 physical checkups, compared with the values measured in the first 5. The cause of this increase seemed to be related to increases in the number of products and the hours of overtime work. Symptoms felt by these workers gradually subsided as they were continuously exposed to TCE for more than 2 or 3 years. Some workers of long service had a tendency toward addiction to TCE. In the legally required environmental monitoring of TCE, and other harmful organic solvents, the 8 workplaces fell into either classification 1 or 2, indicating that the environmental conditions are not harmful. More importantly, the above evaluations did not take into account long hours of overtime put in by those workers. Turning to the checkups of 1,1,1-trichloroethane workers, the results of legally required physical checkups and environmental monitoring were almost the same as those for TCE.(ABSTRACT TRUNCATED AT 250 WORDS)