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8. Utilizzo di un metodo citofluorimetrico per la conta di Listeria monocytogenes nei challenge test

Author

D'Onofrio, F., Schirone, M., Lolli, M. G., Sacchini, F., Krasteva, I., DI Febo, T., Pomilio, F., Centorame, P., Tittarelli, M., Luciani, M., and Iannetti, L.

Subjects
The following study aims to compare Listeria monocytogenes enumeration performed by ISO 11290-2 and flow cytometry. Experiments were carried out on two L. monocytogenes strains grown at 8 and 4°C. The results highlighted that flow cytometry technique did not count the total number of bacterial cells enumerated by the official technique. Nevertheless it was evinced a constant relation between values obtained with both techniques: starting from a value of 4 log CFU/ml, flow cytometry permitted to enumerate 90-95% of the total L. monocytogenes cells counted by ISO 11290-2. This percentage decreased to 85-90% for 5 log CFU/ml, 65-70% for 6 log UFC/ml, The following study aims to compare Listeria monocytogenes enumeration performed by ISO 11290-2 and flow cytometry. Experiments were carried out on two L. monocytogenes strains grown at 8 and 4°C. The results highlighted that flow cytometry technique did not count the total number of bacterial cells enumerated by the official technique. Nevertheless it was evinced a constant relation between values obtained with both techniques: starting from a value of 4 log CFU/ml, flow cytometry permitted to enumerate 90-95% of the total L. monocytogenes cells counted by ISO 11290-2. This percentage decreased to 85-90% for 5 log CFU/ml, 65-70% for 6 log UFC/ml, 60-65% for 7 log CFU/ml, and 55-60% for 8 log CFU/ml. No significant differences of the enumeration were observed as function of strains and growth temperature, and 55-60% for 8 log CFU/ml. No significant differences of the enumeration were observed as function of strains and growth temperature, 60-65% for 7 log CFU/ml
Published
2022

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