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The aim of the research is to evaluate the professional risk of the health workers of a pediatric hospital, mainly devoted to the care of adolescents. A seroepidemiological survey was therefore performed during the preliminary screening of an anti-hepatitis-B vaccination program, implemented in a suburban 150-beds pediatric hospital, with a predominance of elective surgery patients. The prevalence of past or ongoing HBV infection in a sample of adolescent inpatient (10-19 years old) in the same institution was known from an another study (13.2%). The prevalence of any HBV marker among 360 workers was 16.3%, lower in males (13.5 versus 17.7% in females), and in those with an higher level of instruction; the prevalence resulted significantly higher with increasing age and length of hospital service. Out of 321 candidates to anti-HBV vaccination 245 employees accepted the procedure (76.3%), significantly more females than males (80% versus 68.9%) and more subjects less than 30 years-old (84.3% versus 62% of subjects more than 40 years-old subjects). Only 13 subjects (5.3%) did not reach a protective titer after the third dose of the plasma-derived vaccine a protective titer. No major adverse event occurred.