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Your search keyword '"Ciardiello, F"' showing total 6 results
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6 results on '"Ciardiello, F"'

1. Core curriculum. Oncologia clinica

Author

Antonelli, M, Zora, Baretta, Bianco, C, Bianco, R, Bria, E, Cancello, G, Ciardiello, F, Cingarlini, S, Condello, C, Damiano, V, Nullm, nullDe Laurentiis, Nullg, nullDella Vittoria Scarpati, Dieci, Mv, Forestieri, V, Nullg, nullDi Lorenzo, Nullm, nullDi Maio, Guarneri, V, Massari, F, Melisi, D, Morabito, A, Palmieri, G, Pensabene, M, Pepe, S, Perrone, F, Piccirillo, Mc, ARPINO, GRAZIA, CARLOMAGNO, Chiara, BIANCO, ANGELO RAFFAELE, CONTEGIACOMO, ALMA, LAURIA, ROSSELLA, MARINELLI, ALFREDO, MATANO, ELIDE, PERSICO, GIOVANNI, Antonelli M, Arpino G, Zora Baretta, Bianco C, Bianco R, Bria E, Cancello G, Carlomagno C, Bianco AR, Ciardiello F, Cingarlini S, Condello C, Contegiacomo A, Damiano V, De Laurentiis M, Della Vittoria Scarpati G, Dieci MV, Forestieri V, Di Lorenzo G, Di Maio M, Guarneri V, Lauria R, Marinelli A, Massari F, Matano E, Melisi D, Morabito A, Palmieri G, Pensabene M, Pepe S, Perrone F, Persico G, Piccirillo MC, Angelo Raffaele Bianco, Sabino De Placido, Giampaolo Tortora e Pierfranco Conte, Antonelli, M, Arpino, Grazia, Zora, Baretta, Bianco, C, Bianco, Roberto, Bria, E, Cancello, G, Carlomagno, Chiara, Bianco, ANGELO RAFFAELE, Ciardiello, F, Cingarlini, S, Condello, C, Contegiacomo, Alma, Damiano, V, Nullm, nullDe Laurentii, Nullg, nullDella Vittoria Scarpati, Dieci, Mv, Forestieri, Valeria, Nullg, nullDi Lorenzo, Nullm, nullDi Maio, Guarneri, V, Lauria, Rossella, Marinelli, Alfredo, Massari, F, Matano, Elide, Melisi, D, Morabito, A, Palmieri, G, Pensabene, M, Pepe, S, Perrone, F, Persico, Giovanni, Piccirillo, Mc, Lauria E, Bianco, R, and Forestieri, V

Published
2015

4. Manuale di Oncologia Clinica

Author

Bianco, A. R., Biondi, E., Carlomagno, C., Ciardiello, F., Contegiacomo, A., DE LAURENTIIS, M., DE PLACIDO, S., Lauria, R., Marinelli, A., Matano, E., Morabito, A., Palmieri, G., Pepe, S., Perrone, F., Tagliaferri, P., Tortora, Giampaolo, A.R.Bianco, Bianco, ANGELO RAFFAELE, E., Biondi, Carlomagno, Chiara, Ciardiello, Fortunato, Contegiacomo, Alma, DE LAURENTIIS, Michelino, DE PLACIDO, Sabino, Lauria, Rossella, Marinelli, Alfredo, Matano, Elide, A., Morabito, Palmieri, Giovannella, Pepe, Stefano, F., Perrone, P., Tagliaferri, and Tortora, Giampaolo

Subjects
clinica, testo universitario, Oncology, Oncologia, Immunology, Oncology, Immunology, Medicine, Medicine
Abstract

Manuale di Oncologia Clinica rivolto agli studenti delle Facoltà di Medicina e Chirurgia e agli specializzandi in Oncologia Medica

Published
1996

5. Detection of KRAS mutations in colorectal carcinoma patients with an integrated PCR/sequencing and real-time PCR approach

Author

Nicola Normanno, Gerardo Botti, Carmine Pinto, R Vincenzo Iaffaioli, Cristin Roma, Fabiana Tatangelo, Oscar Nappi, Anna Maria Rachiglio, Fortunato Ciardiello, Pietro Carotenuto, Carotenuto, P, Roma, C, Rachiglio, Am, Tatangelo, F, Pinto, C, Ciardiello, Fortunato, Nappi, O, Iaffaioli, Rv, Botti, G, Normanno, N., Carotenuto, P., Roma, C., Rachiglio, A. M., Tatangelo, F., Pinto, C., Ciardiello, F., Nappi, O., Iaffaioli, V., and Botti, G.

Subjects
medicine.drug_class, Colorectal cancer, EGFR, Mutant, Reproducibility of Result, Colorectal Neoplasm, Biology, Adenocarcinoma, Monoclonal antibody, medicine.disease_cause, Sensitivity and Specificity, Cohort Studies, Proto-Oncogene Proteins p21(ras), chemistry.chemical_compound, colorectal carcinoma, Proto-Oncogene Proteins, Genetics, medicine, KRAS, Humans, Neoplasm Metastasis, Gene, Oligonucleotide Array Sequence Analysis, Pharmacology, therapy, Proto-Oncogene Protein, Oligonucleotide Array Sequence Analysi, Reverse Transcriptase Polymerase Chain Reaction, Reproducibility of Results, medicine.disease, ras Protein, Molecular biology, Neoplasm Metastasi, Real-time polymerase chain reaction, chemistry, Mutation, ras Proteins, Molecular Medicine, DNA fragmentation, Cohort Studie, Colorectal Neoplasms, DNA, Human
Abstract

Aims: Patients with metastatic colorectal carcinoma (mCRC) carrying activating mutations of the KRAS gene do not benefit from treatment with anti-EGF receptor monoclonal antibodies. Therefore, KRAS mutation testing of mCRC patients is mandatory in the clinical setting to aid in the choice of appropriate therapy. Materials & methods: We developed a cost-effective approach for the determination of KRAS mutations in codons 12 and 13 in clinical practice based on a sensitive PCR/sequencing technique and the commercially available real-time PCR-based Therascreen® kit (DxS Ltd). Results & conclusion: The PCR/sequencing test was able to detect 10% mutant DNA in a background of wild-type DNA. By using this assay, we determined the mutational status of KRAS in 527 out of 540 (97.6%) formalin-fixed paraffin-embedded tissues from mCRC patients. PCR/sequencing was not conclusive in 13 cases, in which low-intensity peaks suggestive of potential mutations were identified. The DxS assay, which showed a sensitivity of 1%, identified mutations in 11 out of 13 inconclusive cases. Interestingly, five of these 11 cases showed high levels of DNA fragmentation. No significant difference was found in the ability of PCR/sequencing and DxS to identify KRAS mutations within 160 cases with more than 30% tumor cells. However, in 24 samples with less than 30% tumor cells, DxS showed an higher sensitivity. In conclusion, our findings suggest that PCR/sequencing can be used for mutational analysis of the majority of tumor samples that have more than 30% tumor cell content, whereas more sensitive and expensive tests should be reserved for inconclusive cases and for samples with a low amount of tumor cells.

Published
2011

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