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12 results on '"Brocchi, E."'

2. Profilassi vaccinale della malattia di Newcastle e della Rinotracheite del tacchino: somministrazione simultanea di vaccini vivi ad un giorno di vita

Author

Cecchinato M., Muñoz O., Terregino C., Cecchettin K., LUPINI, CATERINA, LISTORTI, VALERIA, CATELLI, ELENA, MONINI M., BABSA S., RUGGIERI F.M., LAVAZZA A., CORDIOLI P., BROCCHI E., Cecchinato M., Lupini C., Listorti V., Muñoz O., Terregino C., Cecchettin K., and Catelli E.

Subjects
RINOTRACHEITE DEL TACCHINO, VACCINI, Newcastle disease virus, Avian metapneumovirus, MALATTIA DI NEWCASTLE, vaccination
Abstract

Per la Malattia di Newcastle (ND) e la Rinotracheite del tacchino (TRT), vaccini vivi attenuati vengono comunemente somministrati nei primi giorni di vita ad almeno una settimana di distanza l’uno dall’altro per il timore che si verifichino interferenze tra virus vaccinali. La possibilità di associare in incubatoio tali interventi avrebbe numerosi vantaggi sia di ordine pratico, che economico e sanitario. In quest’ottica diventa fondamentale assicurarsi che non ci siano interferenze negative tali da compromettere l'efficacia delle vaccinazioni o addirittura causare effetti patologici indesiderati. L’obiettivo del presente lavoro è stato valutare, in condizioni sperimentali, l'interferenza fra due diversi ceppi vaccinali di NDV e uno di Metapneumovirus aviare (AMPV), agente della TRT, somministrati nel tacchino in associazione ad un giorno di vita, secondo un possibile schema vaccinale praticabile in incubatoio. La prova è stata eseguita in condizioni di isolamento biologico in isolatori per pollame. Ottanta tacchinotti commerciali sono stati suddivisi in gruppi e vaccinati al primo giorno di vita per AMPV col ceppo VCO3, e per NDV con i ceppi B1 o VG/GA, somministrati singolarmente o in associazione (VCO3 + B1 o VCO3+VG/GA). Nei 30 giorni successivi, ad intervalli stabiliti, è stata valutata l’eliminazione respiratoria dei virus vaccinali mediante real time RT-PCR, e la risposta anticorpale umorale per NDV ed AMPV, mediante inibizione dell’emoagglutinazione ed ELISA, rispettivamente. Infine, parte dei soggetti vaccinati per AMPV, singolarmente o in associazione, sono stati infettati sperimentalmente con AMPV allo scopo di valutare la protezione immunitaria mediante misurazione della sintomatologia clinica. I risultati ottenuti permettono di concludere che la somministrazione al tacchino di un giorno di vita di vaccino vivo attenuato AMPV ceppo VC03, in associazione con i vaccini vivi NDV sia ceppo B1 sia ceppo VG/GA, conferisce protezione al challenge con AMPV sovrapponibile a quella evocata dal vaccino somministrato singolarmente, e non determina interferenze negative sulla replicazione dei virus vaccinali a livello respiratorio e sulla risposta immunitaria umorale. Resta da verificare se la protezione nei riguardi di NDV a seguito di vaccinazione combinata sia sovrapponibile a quella ottenibile con la vaccinazione singola.

Published
2011

3. [Dietetic considerations of the peptic ulcer treatment (author's transl)].

Author

Bianchi V, Casini M, Bortolan S, Rosa P, Mazzoni M, Brocchi E, and Gremigni M

Subjects
Acute Disease, Animals, Dietary Fiber, Dietary Proteins therapeutic use, Food Service, Hospital standards, Gastric Acid metabolism, Hospitalization, Humans, Mastication, Milk, Peptic Ulcer prevention & control, Peptic Ulcer therapy, Physical Exertion, United States, Peptic Ulcer diet therapy
Abstract

Stated that the alimentary inconguities, the improper mastication and the quickly eaten of foods are to avoid for the direct irritant effect on the stomach, the authors have examinate the various dietetic treatments, imploied for peptic ulcer and they conclude that only during the acute clinical phase more restrictive regimens are necessary. When the patient in on way of recovery, the alimentation must be free in agreement to the tolerance and personal taste using more variety of food. Moreover it's necessary to remember that the patient is not an "ulcer", but "his ulcer" be treated with the psycho-pathological implication correlated.

Published
1980

4. [Use of the ELISA (enzyme-linked immunosorbent assay) method in detection of anti-toxoplasma IgG and IgM].

Author

Brocchi E, Genzini E, Valcavi P, and Schito GC

Subjects
Agglutination Tests, Complement Fixation Tests, Fluorescent Antibody Technique, Humans, Toxoplasmosis immunology, Enzyme-Linked Immunosorbent Assay, Immunoenzyme Techniques, Immunoglobulin E analysis, Immunoglobulin M analysis, Toxoplasmosis diagnosis
Abstract

The enzyme-linked immunosorbent assay (ELISA) has been applied for detecting anti-toxoplasma serum IgG and IgM. ELISA was carried out with alkaline phosphatase-labeled anti-IgG and anti-IgM antibodies. Both conjugates specificity was investigated by sucrose density gradient fractionating of an immunofluorescence positive serum and by ELISA testing of all the fractions for IgG and IgM. A good agreement of ELISA results from 400 sera was found when they were compared with others obtained by several serological methods: direct agglutination, complement fixation, IgG/IgM-IIF. Occasionally false-positive IgM-ELISA and IgM-IIF results were observed, probably due to non specific IgM antibodies. Absorption of sera with whole parasites resulted in a removal of specific IgM, whilst false-positive IgM-ELISA reactions were unaffected. ELISA procedure is simple and rapid to carry out in a large scale and it seems to be for routine purposes an adequate substitute for toxoplasmosis IIF test. The possibility to quantitate ELISA results would give the opportunity to standardize the test not only in one, but even among several laboratories.

Published
1981

5. [Application of recent methods to the rapid diagnosis of various viral diseases].

Author

Genzini E, Brocchi E, and Valcavi P

Subjects
Antibodies, Viral analysis, Complement Fixation Tests, Enzyme-Linked Immunosorbent Assay, Hemagglutination Inhibition Tests, Hemolytic Plaque Technique, Humans, Immunologic Techniques, Virus Diseases diagnosis
Abstract

Three new rapid immunological tests (ELISA, hemolisis-in-gel, single-radial-complement-fixation) have been applied for detecting antibodies in 200 sera against widespread virus infections. Results were compared with those of complement fixation and hemagglutination inhibition tests and good correlation was found. The new tests are simple to perform, rapid, sensitive and could be well suited for screening of large number of serum samples.

Published
1980

6. [Esophageal ulcer subsequent to endoscopic sclerosis of varices. Prophylaxis and therapy with sodium alginate].

Author

Caletti GC, Brocchi E, Guizzardi G, Zani L, Foti A, Bartolucci C, Rudelli G, Dal Prà ML, and Labò G

Subjects
Adult, Aged, Esophageal Diseases etiology, Female, Glucuronic Acid, Hexuronic Acids, Humans, Male, Middle Aged, Prospective Studies, Ulcer drug therapy, Ulcer etiology, Alginates therapeutic use, Esophageal Diseases drug therapy, Esophageal and Gastric Varices therapy, Sclerosing Solutions adverse effects
Published
1986

8. [Endoscopic treatment of obstructive cholangitis].

Author

Caletti GC, Verucchi G, Vitolo A, Brocchi E, Bolondi L, and Labò G

Subjects
Aged, Cholangitis surgery, Cholangitis therapy, Cholestasis therapy, Drainage, Humans, Male, Preoperative Care, Cholangitis complications, Cholestasis etiology, Endoscopy, Gallstones complications
Abstract

A long catheter can be positioned in the bile ducts by means of a fibre glass gastroduodenoscope, thus making it possible to drain bile and inject medicaments locally. This technique gives excellent results and is particularly indicated in cases of choledocic lithiasis complicated by cholangitis in order to clear up the infection and send the patient in for surgery in ideal condition.

Published
1980

9. Physiopathological aspects of the empty sella syndrome combined with prolactin hypersecretion.

Author

Bianchi V, Casini M, Grosso A, Bortolan S, and Brocchi E

Subjects
Adenoma diagnostic imaging, Adult, Amenorrhea physiopathology, Diagnosis, Differential, Empty Sella Syndrome diagnostic imaging, Female, Galactorrhea physiopathology, Humans, Pituitary Neoplasms diagnostic imaging, Pregnancy, Tomography, X-Ray Computed, Empty Sella Syndrome physiopathology, Prolactin blood
Published
1980

10. [The primary empty sella syndrome. A case report study (author's transl)].

Author

Casini M, Bianchi V, Brocchi E, Bortolan S, and Rosa P

Subjects
Adult, Cerebrospinal Fluid Rhinorrhea etiology, Diagnosis, Differential, Empty Sella Syndrome diagnostic imaging, Empty Sella Syndrome etiology, Headache etiology, Humans, Hypopituitarism etiology, Male, Radiography, Diabetes Complications, Empty Sella Syndrome complications
Abstract

The empty sella results from an extension of the subarachnoid space into an intrasellar position with subsequent remodeling of the sella turcica and the flattening of the pituitary gland. The sella turcica is usually enlarged causing the greatest diagnostic difficulty to distinguish it from a pituitary tumor. The most patients with this syndrome usually have normal pituitary function, while about 30% have varying degrees of hypopituitarism. The Authors describe a case report of a man with primary empty sella syndrome come to the medical observation for obesity and hyperglyccemia. The endocrine evaluation performed (TRH test, GN-RH test, T3, T4, FT3, FT4, Arginine test, metyrapone test) were normal. OGTT shows a maturity onset diabetes (glycemia = 160 mg%). This rare clinical association is not well understood. Probably this diabetes is to the interruption of the normal hypothalamo-pituitary neurovascular connection.

Published
1980

11. [Therapeutic effect of pirenzepine in chronic superficial gastritis in patients with portal hypertension and liver cirrhosis].

Author

Caletti GC, Brocchi E, Zani L, Valentini P, Guizzardi G, Grilli T, and Bartolucci C

Subjects
Adult, Aged, Chronic Disease, Clinical Trials as Topic, Female, Gastritis etiology, Humans, Male, Middle Aged, Pirenzepine, Benzodiazepinones therapeutic use, Gastritis drug therapy, Hypertension, Portal complications, Liver Cirrhosis complications, Parasympatholytics therapeutic use
Published
1985

12. [Endoscopic diagnosis and therapy of choledocho-duodenal fistulas].

Author

Caletti GC, Brocchi E, Guizzardi G, Passaniti A, Zani L, Bartolucci C, and Labò G

Subjects
Biliary Fistula surgery, Common Bile Duct Diseases surgery, Duodenal Diseases surgery, Humans, Intestinal Fistula surgery, Biliary Fistula diagnosis, Common Bile Duct Diseases diagnosis, Duodenal Diseases diagnosis, Endoscopy, Intestinal Fistula diagnosis
Published
1983

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