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Shallots are bulb-shaped commodities that are difficult to maintain in new conditions because they are easily damaged. An effective effort to prevent deterioration of food quality during storage is by coating it using nano chitosan. The effectiveness of nano chitosan is influenced by particle size which related to the ratio of chitosan and STPP (sodium tripolyphosphate). This study aimed to determine the effect of nano chitosan coating with the addition of different ratios of chitosan and STPP and the appropriate ratio of chitosan and STPP on nano chitosan as a coating to maintain the quality of shallot bulbs (Allium ascalonicum L. var. Bauji). This study used a completely randomized design (CRD) method with four treatments and five replications. The treatments of this research were P0= Control, PI= Nano chitosan ratio of chitosan: STPP 1:3, P2= Nano chitosan ratio of chitosan:STPP 1:4, and P3= Nano chitosan ratio of chitosan:STPP 1:5. The variables of this study were the percentage of damage, the percentage of diameter shrinkage, weight loss, color, hardness, and moisture content. Data were analyzed using the ANOVA test and continued with the DMRT test. The application of nano chitosan coating on shallot bulbs could reduce damage, shrinkage of tuber diameter, weight loss, color, hardness, and decrease in water content better than the control treatment. The best results were shown by treatment P3 (1:5) with a percentage of damage of 8%, diameter shrinkage of 20.20%, weight loss of 18.40%, total color change of 54.45, hardness of 226.23 N, and a decrease in water content of 4.65% at the final water content of 79.09%.

Honey is a natural sugar produced by bees from the nectar of plants or secretions of living parts of plants or excrtions of plant sucking insects on the living parts of plants. Pollen is an important component contained in honey, as a source of protein for bees. The This study aims to determine the diversity of pollen which is a source of bee feed, and knowing the type of honey based on the type and frequency of pollen in honey in 5 villages in Boyolali Regency. The method used for the pollen analysis is acetolysis. The quantitative data of pollen analysis using Excel Software. The results showed the diversity of plant pollen in honey from 5 village in Boyolali is 52 taxa, and the type of honey based on the frequency and types of pollen varies, the village Cerme and Cepogo honey is multifloral honey, Kiringan and Pager Village honey including monofloral honey, and Kemiri Village honey is bifloral honey.

Malabar plum [Syzygium jambos (L.) Alston.] is a tropical plant which is used as a medicinal plant, because it contains secondary metabolites, especially in the leaves. The different leaves developmental stages can affect physiological changes, especially metabolic processes, so it is suspected to affect the antioxidant content and activity. The objective of this research was to study the difference of leaves morphology, chlorophyll contents, antioxidant contents, and activity at the different leaves developmental stages. Samples were taken from Kaliboto Village, Purworejo, Central Java. The leaf color measurement was using colorimetry; determination of chlorophyll, carotenoids, ascorbic acid, and total phenol content is was using spectrophotometry; and antioxidant activity was using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Qualitative data were analyzed descriptively, while quantitative data were analyzed by ANOVA tests. The results showed that the higher level of leaves development, the higher pigment content, total phenol, and antioxidant activity ascorbic acid content in the mature leaves is lower when compared to the young and old leaves. The results of this research provide the information that can support the use of Malabar plum leaves in traditional medicinal activity and pharmaceutical industry, as well as basic information for plant breeding.

Soybean plant [Glycine max (L.) Merr.] Is a food crop that is a source of fat, protein, and minerals. Drought stress is a major obstacle in the cultivation of soybean plants, which causes the synthesis of excessive amounts of Reactive Oxygen Species (ROS) which triggers oxidative stress. Hydrogen peroxide (H2O2) is the most stable ROS, in low concentrations it can act as a signaling molecule and activate an antioxidant defense system to protect plants from oxidative stress. The study was conducted at the Plant Structure and Function Biology Laboratory of Diponegoro University. The study aims to determine the effect of H2O2 treatment on the condition of different levels of water availability on the vegetative growth of soybean plants variety Deja 1. Research using a completely randomized design (CRD) 4x2 factorial pattern. The first factor is H2O2 concentration (0 0.5 1 and 2 mM). The second factor is the level of water availability (100 and 35% of field capacity). Each treatment was repeated 5 times. Quantitative data were analyzed by Analysis of Variance (ANOVA) followed by DMRT test at 95% confidence level. The results showed that there was an interaction between the H2O2 concentration factor and the level of water supply to the canopy fresh weight. The 2 mM H2O2 treatment at 100% water availability and 1 mM H2O2 with 35% water availability increased vegetative growth as indicated by the canopy and root fresh weight parameters, number of leaves, stomata opening width, relative water content. Based on the results of the study it can be stated that the exogenous H2O2 treatment of leaves with certain concentrations at all different levels of water availability can increase the vegetatif growth of soybean variety of Deja 1.

Tawangmangu tangerine (Citrus reticulate Blanco subsp. tawangmangu) is one of prime local fruit from Tawangmangu region, Karanganyar, Central Java. This tangerine have good appearance, easy to peeled, sweet flavored fruit, and high production rate. However at 1984 Tawangmangu tangerine run into depreciation of population caused by CVPD (Citrus Vein Phloem Degeneration). As a result, this variety listed as endangeredThe effort of consevation by culture tissue technique can order to obtain prime seeds that free from virus or disease. This study was aimed to understand the effect of IBA and BAP in culture medium on growth of apical and lateral shoot of Tawangmangu tangerine. The treatment provided were IBA (0 ppm, 0.5 ppm, and 1 ppm) and BAP (0 ppm, 1 ppm and 2 ppm). The treatment in this research was using complete randomized factorial design in 9 different treatment. This research resulted that added combination of IBA and BAP affecting growth of Tawangmangu tangerine but the interaction of IBA and BAP did not happen. The highest shoot length occurs on treatment IBA 1 ppm+BAP 2 ppm while when the fastest shoot appears occurs on treatment IBA 0 ppm+ BAP 2 ppm and the highest shoot formed on treatment IBA 0.5 ppm+BAP 2 ppm. Benefit of the research Efforts to conserve Tawangmangu orange plants need to be done to maintain the diversity of germplasm and support the cultivation in a manner. One technique that can support the conservation of plants is by tissue culture techniques.

Kaili is one of the ethnic region in Central Sulawesi which saves a lot of cultural values and traditions. As a traditional community, their life is very dependent upon natural resources contained in the environment. They still have knowledge, traditional culture, treatment and utilization system against various types of plants. The purpose of the study was to examine the knowledge understanding and utilization of medicinal plants by local community Tompu District of Kaili. Data knowledge and utilization were collected through interview, literature study, exploratory survey methods, PEA (participatory ethnobotanical appraisal), questionnaire and from interviews with the informants. The results from interviews showed that of public knowledge is still based on the traditional concept. Based on the results identifications obtained by (90 species). As many as six species medicinal plants to often used the Tompu community are Euphorbia hirta L. Phyllanthus niruri L. Ageratum L. Blumea conyzoides balsaminifera L. (DC). Kleinhovia hospita L and Tabernaemontana pandacaqui. The benefits of this research to the development of science is expected to be complete scientific data regarding the utilization of medicinal plants natural resources on Tribal society Kaili in Tompu.How to CiteIfandi, S., Jumari, J., & Suedy, S. W. A. (2016). Knowledge Understanding and Utilization of Medicinal Plants by Local Community Tompu District of Kaili, Sigi Biromaru, Central Sulawesi. Biosaintifika: Journal of Biology & Biology Education, 8(1), 1-11.

Palynological evidences (pollen and spores) have been used to study mangrove diversity and vegetation fluctuation at coastal zone sites at Muktiharja, Kemijen Semarang , province of Central Java. Data was collected in April 2nd to April 17th 2006 following transect method, for sediments sampling used surface subsurface sampling method (depth in 25cm). Pollen preparation used Acetolysis method. The result showed that mangrove forest in the coastal zone of Muktiharja, Kemijen Semarang was dominated by Rhizophoarceae pollen type (included Rhizophora genus : Rhizophora mucronata, Rhizophora stylosa dan Rhizophora apiculata). Vegetation of mangrove forrest in this bay was fluctuated which terrestrial climate was influenced in this mangrove forrest.

Pollen and spore fossil of Pliocene sedimentary (Tapak Formation) have been used as a guidance for prediction (bioprediction) of climate change which happened at Pliocene age in Banyumas. Geomorphological and vegetation changes happenin conformity with climate changes. By knowing pollen and shpore fossils, we can know thetype of vegetation whichproduce it. Then pollen and shprore fossils which found widelyon the sedimentary rock is an exact way for tracing of climate change which had happened. The aimof this research is to explore bioprediction method base on polen andsphore data, to know morphological change which happened because of climate change on Plioceneage in Banyumas. This research consist of field and labolatory work. Field work is for taking rock sample andmaking stratigraphic collumn. Labolatory work consist of making plate from the samples using asetolisis methode, identification and clasification of fosils and palynology analisis. The result of the research show that the research areacan be included on the zone ofPodocarpus imbricatusfrom Late Pliocene Age which is shown by presence ofPodocarpus imbricatusandStenochlaenidites papuanus.There has 3 events of climate change that are hot-cold-hot which corelate withtransgresion (relative sea level rise) andregresion (relative sea level drop)