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Abstrak: Penelitian ini bertujuan untuk mengevaluasi implementasi Sistem Penjaminan Mutu Internal Perguruan Tinggi (SPMI-PT) Politeknik Negeri Manado yang mengacu pada Sistem Manajemen Mutu (SMM) ISO 9001, mengidentifikasi profil budaya organisasi Politeknik Negeri Mando, dan menganalisis hubungan antara implementasi SMM ISO 9001 dan budaya organisasi institusi. Penelitian menggunakan pendekatan kuantitatif dengan instrumen kuesioner. Penelitian menemukan bahwa implementasi delapan prinsip mutu ISO 9001 belum mencapai tingkat efektivitas yang maksimal dan tipe budaya clan mendominasi profil budaya organisasi institusi. Selain itu, juga ditemukan bahwa budaya clan berkorelasi signifikan dengan prinsip mutu kepemimpinan, keterlibatan orang-orang dalam aktivitas institusi, pendekatan proses dalam aktivitas institusi, pendekatan fakta untuk pengambilan keputusan, dan prinisp mutu bubungan yang saling menguntungkan; budaya market berkorelasi signifikan dengan prinsip mutu fokus pada pelanggan; budaya hierarchy berkorelasi signifikan dengan prinsip mutu pendekatan sistem untuk manajemen institusi, dan perbaikan terus-menerus. Hasil penelitian menjadi dasar bagi institusi untuk dapat mengembangkan budaya organisasi yang kuat sebagai pendukung dalam menerapkan SPMI-PTsecara efektif dan meningkat berkelanjutan. Keywords: sistem penjaminan mutu internal, budaya organisasi, perguruan tinggi IDENTIFICATION OF THE ORGANIZATIONAL CULTURE PROFILE SUPPORTING THE IMPLEMENTATION OF THE HIGHER EDUCATION QUALITY ASSURANCE SYSTEM Abstract: This research was aimed to evaluate the implementation of the Internal Quality Assurance System of the Higher Education (SPMI-PT) of Manado State Polytechnic based on the ISO 9001 Quality Management System (QMS), to identify the organizational culture profile of Manado State Polytechnic, and to examine the relationship between the implementation of ISO 9001 and the culture profile of the institution. Questionnaireswere used in order to achieve the research aims.The study found that the implementation of the eight ISO 9001 principles had not achieved the maximum level of effectiveness, and Clan culture dominated the profile of the organizational culture of Manado State Polytechnic.The research also found that: Clan culture was significantly correlated with the quality principles of Leadership, People Involvement, Process Approach, Factual Approach to Decision Making, and Mutually Beneficial Supplier Relationships;Market culture was significantly correlated with the quality principle of Customer Focus while Hierarchy culture wassignificantly correlated with the quality principles of Systems Approach to Management,and Continual Improvement. The findings are the basis for the institution to be able to develop a strong organizational culture as a driver in implementing the SPMI-PT effectively and sustainably. Keywords: quality assurance system, organizational culture, higher education