1. [Metabolome analysis of solid tumors].
- Author
-
Budczies J
- Subjects
- 4-Aminobutyrate Transaminase metabolism, Breast pathology, Breast Neoplasms pathology, Colonic Neoplasms drug therapy, Colonic Neoplasms pathology, Female, Gas Chromatography-Mass Spectrometry, Glutamic Acid metabolism, Glutaminase antagonists & inhibitors, Glutamine metabolism, Humans, Metabolome drug effects, Neoplasms drug therapy, Neoplasms, Hormone-Dependent drug therapy, Neoplasms, Hormone-Dependent pathology, Ovarian Neoplasms pathology, Ovary pathology, beta-Alanine metabolism, Biomarkers, Tumor, Metabolome physiology, Neoplasms pathology
- Abstract
Metabolomics, the newest of the omics sciences that also include genomics, transcriptomics and proteomics, has matured into a reliable high-throughput technology. Gas chromatography combined with time-of-flight mass spectrometry (GC-TOFMS) is a suitable method to analyze the central metabolism in fresh frozen tumor tissue samples. Bioinformatics methods, including the PROFILE clustering developed by us, permit integrated analysis and fast interpretation of metabolomics data in the context of enzymatic reactions and metabolic pathways. The metabolome analyses of three solid tumor types presented here, together with the results of other authors, show that metabolites are suitable as biomarkers and provide diverse options for translation into the clinical setting.
- Published
- 2016
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