1. Influence of oxygen tension on CD133 phenotype in human glioma cell cultures
- Author
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François Berger, Shi Yong Liu, François M. Vallette, Michèle El Atifi, Nadine Platet, Lisa Oliver, Didier Wion, Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Recherches en cancérologie, Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Ligue contre le Cancer (comité Isère), and Issartel, Jean-Paul
- Subjects
Cancer Research ,Human glioma ,MESH: Cell Hypoxia ,MESH: Flow Cytometry ,MESH: Dose-Response Relationship, Drug ,MESH: Glioma ,0302 clinical medicine ,glioma ,MESH: Reverse Transcriptase Polymerase Chain Reaction ,AC133 Antigen ,CD133 ,MESH: Antigens, CD ,0303 health sciences ,Chemistry ,Reverse Transcriptase Polymerase Chain Reaction ,MESH: Peptides ,MESH: Gene Expression Regulation, Neoplastic ,Flow Cytometry ,Phenotype ,Immunohistochemistry ,Cell Hypoxia ,Oxygen tension ,Cell biology ,Human tumor ,Gene Expression Regulation, Neoplastic ,Oncology ,030220 oncology & carcinogenesis ,[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,Oxygen level ,MESH: Oxygen ,cancer stem cell ,MESH: Cell Line, Tumor ,MESH: Glycoproteins ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,03 medical and health sciences ,[SDV.CAN] Life Sciences [q-bio]/Cancer ,Cancer stem cell ,Antigens, CD ,Glioma ,Cell Line, Tumor ,medicine ,Humans ,[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,030304 developmental biology ,Glycoproteins ,cell culture ,MESH: Humans ,Dose-Response Relationship, Drug ,MESH: Immunohistochemistry ,medicine.disease ,Molecular biology ,Oxygen ,Cell culture ,Peptides - Abstract
International audience; Under standard culture conditions, tumor cells are exposed to 20% O(2), whereas the mean tumor oxygen levels within the tumor are much lower. We demonstrate, using low-passaged human tumor cell cultures established from glioma, that a reduction in the oxygen level in these cell cultures dramatically increases the percentage of CD133 expressing cells.
- Published
- 2007