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3 results on '"NIH 3T3 Cells"'

1. Morphologisches Spektrum USP6-rearrangierter Läsionen.

Author

Mechtersheimer, G. and Werner, M.

Abstract

Copyright of Der Pathologe is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2018
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2. Gadd45β is a pro-survival factor associated with stress-resistant tumors

Author

A Engelmann, G W Bornkamm, Wolfgang Deppert, C Stocking, and Daniel Speidel

Subjects
Cancer Research, Lymphoma, B-Cell, Tumor suppressor gene, Survival, Mice, SCID, Biology, medicine.disease_cause, Mice, Mice, Inbred NOD, Genetics, medicine, Animals, Molecular Biology, B-cell lymphoma, MYC, p53, Bcl-x(L), retroviral insertional mutagenesis, stress-resistance, medicine.disease, Antigens, Differentiation, Lymphoma, Transplantation, Mice, Inbred C57BL, Disease Models, Animal, Oxidative Stress, Tumor progression, Immunology, Cancer research, NIH 3T3 Cells, Syngenic, GADD45B, Carcinogenesis, Ex vivo
Abstract

Tumors that acquire resistance against death stimuli constitute a severe problem in the context of cancer therapy. To determine genetic alterations that favor the development of stress-resistant tumors in vivo, we took advantage of polyclonal tumors generated after retroviral infection of newborn Elambda-MYC mice, in which the retroviral integration acts as a mutagen to enhance tumor progression. Tumor cells were cultivated ex vivo and exposed to gamma-irradiation prior to their transplantation into syngenic recipients, thereby providing a strong selective pressure for pro-survival mutations. Secondary tumors developing from stress-resistant tumor stem cells were analysed for retroviral integration sites to reveal candidate genes whose dysregulation confer survival. In addition to the gene encoding the antiapoptotic Bcl-x(L) protein, we identified the gadd45b locus to be a novel common integration site in these tumors, leading to enhanced expression. In accord with a thus far undocumented role of Gadd45beta in tumorigenesis, we showed that NIH3T3 cells overexpressing Gadd45beta form tumors in NOD/SCID mice. Interestingly and differently to other known 'classical' antiapoptotic factors, high Gadd45beta levels did not protect against MYC-, UV- or gamma-irradiation-induced apoptosis, but conferred a strong and specific survival advantage to serum withdrawal.

Published
2008

3. [Morphological spectrum of USP6 rearranged lesions].

Author

Mechtersheimer G and Werner M

Subjects
Animals, Proto-Oncogene Proteins, Ubiquitin Thiolesterase, Bone Cysts, Aneurysmal, Fasciitis
Abstract

USP6, also known as Tre-2 and TRE17, is an ubiquitase-specific proteinase that was identified more than two decades ago as a potential oncogene when it exhibited transforming properties upon overexpression in NIH 3T3 cells. Until recently, however, little was known about the function and the oncogenetic activation of USP6. The identification of rearrangements of the USP6 gene in aneurysmal bone cyst and in nodular fasciitis has not only led to a better understanding of the pathogenesis of these entities, but is also a useful tool in their diagnosis and differential diagnostic delineation from morphological mimics. In this review, the clinical, pathomorphological, and molecular genetic aspects of aneurysmal bone cyst and of nodular fasciitis, as well as from related lesions, are presented and discussed.

Published
2018
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