1. [Detection of leptospira in the vitreous body of horses without ocular diseases and of horses with equine recurrent uveitis (ERU) using transmission-electron microscopy].
- Author
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Niedermaier G, Wollanke B, Hoffmann R, Brem S, and Gerhards H
- Subjects
- Animals, Case-Control Studies, Eye Infections, Bacterial diagnosis, Eye Infections, Bacterial microbiology, Female, Horse Diseases microbiology, Horses, Leptospira isolation & purification, Leptospirosis diagnosis, Leptospirosis microbiology, Male, Microscopy, Electron, Transmission methods, Microscopy, Electron, Transmission veterinary, Recurrence, Treatment Outcome, Uveitis diagnosis, Uveitis microbiology, Vitrectomy methods, Vitrectomy veterinary, Vitreous Body surgery, Vitreous Body ultrastructure, Eye Infections, Bacterial veterinary, Horse Diseases diagnosis, Leptospira ultrastructure, Leptospirosis veterinary, Uveitis veterinary, Vitreous Body microbiology
- Abstract
Equine recurrent uveitis (ERU) is caused by persistent intraocular leptospira, which appear to use the vitreous body as a refuge. The detection of leptospira in the vitreous body of horses with spontaneous ERU by histological methods has not yet been described. Thirty eight vitreous body samples from 36 horses with ERU (collected during vitrectomy), and 10 vitreous body samples obtained from 5 horses without ocular disease (control group) were examined by transmission electron microscopy. Prior to sample collection, 2 ml of a leptospira culture suspension were injected into the vitreous body of 2 eyes enucleated from horses of the control group. The detection of leptospira in samples, experimentally inoculated with these bacteria was uncomplicated; in vitreous body samples from horses with spontaneous ERU the detection was successful in only a few cases (3/38). The morphologically varying envelope of leptospira in vitreous body samples of horses which developed ERU spontaneously suggests the existence of a bacterial masquerade in vivo.
- Published
- 2006