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DNA-estimations, done by flow-through-cytophotometry, were performed in 18 malignant tumors of testis. Aneuploid stemlines which are specific for cancers were found in 17 cases. The only exception was a so called "occult seminoma". It showed only a small difference between the stem-line of tumor and normal cells of testis. So, by using DNA-estimation, it was possible to make a correct diagnosis all 18 patients, suffering from tumor of the testis. The aneuploid stem-lines of seminomas did not differ from those of other cancers. The type of proliferative behavior seems to be a criterion for prognosis. Lymphatic nodes with and without formation of metastases very clearly showed differences in the DNA-content of their nuclei.

Difficulties in morphologic classification frequently arise in distinguishing between non-Hodgkin lymphoma and lymph node metastases from anaplastic tumors, mainly germ cell tumors and small cell carcinomas. The present study explores the role of ultrastructural features as a supplement to light microscopy in 14 cases of metastatic disease and 6 non-Hodgkin lymphomas. It appeared that the ultrastructure of the nucleoli, the arrangement of the nuclear bodies and the shape of nuclear invaginations are ultrastructural cell characteristics, which may contribute to the definitive diagnosis.