1. Identification of teleost Thy-1 and association with the microdomain/lipid raft reggie proteins in regenerating CNS axons
- Author
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Michael Przybylski, Soeren-Oliver Deininger, Harald Illges, Lawrence Rajendran, Claudia A. O. Stuermer, Alexander Reuter, Friedrich Lottspeich, University of Zurich, and Stuermer, C A O
- Subjects
Central Nervous System ,Fish Proteins ,Retinal Ganglion Cells ,DNA, Complementary ,Growth Cones ,Molecular Sequence Data ,2804 Cellular and Molecular Neuroscience ,610 Medicine & health ,Nerve Tissue Proteins ,Biology ,1307 Cell Biology ,Cellular and Molecular Neuroscience ,Membrane Microdomains ,Antigen ,Goldfish ,Sequence Homology, Nucleic Acid ,ddc:570 ,1312 Molecular Biology ,medicine ,Animals ,Axon ,Molecular Biology ,Lipid raft ,Cells, Cultured ,Sequence Homology, Amino Acid ,Cell growth ,Lipid microdomain ,Membrane Proteins ,Colocalization ,11359 Institute for Regenerative Medicine (IREM) ,Cell Biology ,Raft ,Fibroblasts ,Axons ,Nerve Regeneration ,Cell biology ,medicine.anatomical_structure ,Thy-1 Antigens ,Intracellular - Abstract
During regeneration, retinal ganglion cell axons in fish upregulate a cell surface protein that is recognized by the monoclonal antibody (mAB) M802. M802 antigen appeared to be linked to the intracellular, membrane-associated lipid raft/microdomain proteins reggie-1 and reggie-2 that were previously shown to be reexpressed in axon-regenerating neurons [Development 124 (1997), 577]. Here, we report the isolation of the M802 antigen and its identification as the teleost homolog of mammalian Thy-1. Fish Thy-1 is detected in the same detergent-insoluble lipid raft fractions from a fibroblast cell line and from axon regenerating retinae as reggie-1 and 2. Importantly, mAB M802 coimmunoprecipitates reggie-1 and 2 from this lipid raft fraction, implying that fish Thy-1 and reggies interact. This correlates with their colocalization in growing cell processes after M802 antigen/Thy-1 activation with mAB M802. These findings suggest a role of clustered M802 antigen/Thy-1 in reggie raft microdomains for cell growth and axon regeneration.
- Published
- 2003