1. [Macromolecular biochemistry of normal and pathological white blood cells in man].
- Author
-
Seeber S
- Subjects
- Hodgkin Disease analysis, Hodgkin Disease pathology, Humans, In Vitro Techniques, Isotope Labeling, Leukemia pathology, Macromolecular Substances analysis, Phosphorus Radioisotopes, RNA, Neoplasm analysis, RNA, Ribosomal analysis, Leukemia analysis, Leukocytes analysis
- Abstract
Leucocytes from normal donors and leukemia patients were isolated and lebelled in vitro with 32P-orthophosphate in order to compare labelling characteristics of nuclear high-molecular weight RNA, labelling characteristics, nucleotide compositions and oligonucleotide frequencies of ribosomal 28 S RNA. These studies revealed 1. structural microheterogeneity of 28 S RNA between the various leukemia cells studies without presenting a leukemia-specific structural marker, 2. an impaired production of ribosomal 28 S RNA from its nuclear precursor 45 S RNA in acute myeloblastic leukemia compared to PHA-stimulated normal lymphocytes. In the second part of this work, the influence of RNA from immunocompetent lymphocytes on the PHA-stimulation of M. Hodgkin lymphocytes was analyzed; the third part deals with studies on macromolecular carriers forcytostatic anthracyclines in human leukemia cells.
- Published
- 1977