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Start Over Author "Janusz, M." Language german
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2. [Rule of antibody structure: the primary structure of a human monoclonal IgA1-immunoglobulin (myeloma protein Tro), II. Cleavage of the monomer IgA-molecule and the reduced and alkylated H- and L-chains by cyanogen bromide (author's transl)].

Author

Ruban E, Starościk K, Janusz M, Kratzin H, and Hilschmann N

Subjects
Amino Acid Sequence, Amino Acids analysis, Cyanogen Bromide, Humans, Macromolecular Substances, Multiple Myeloma immunology, Peptide Fragments analysis, Immunoglobulin A, Immunoglobulin Heavy Chains, Immunoglobulin Light Chains, Myeloma Proteins
Abstract

The monomer of myeloma protein Tro as well as the reduced and alkylated H- and L-chains were cleaved by cyanogen bromide. All cyanogen-bromide fragments were isolated and characterized by amino acid analyses, end-group and molecular weight determinations. The 4 smaller fragments of the 5 H-chain fragments were split with trypsin. The peptides were isolated and their primary structure was determined.

Published
1978

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