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From fecal flora of 66 infants aged 3 to 220 days, 29 children aged 4 to 6 years, 42 adults aged 20 to 64 years and 21 senile persons aged 65 to 86 years, the Lactobacillus flora was analysed qualitatively and quantitatively. In the feces of babies only the resident lactobacilli (L. acidophilus, L. salivarius and L. fermenti) were found, while in the feces of children, adults and senile persons the resident lactobacilli as well as the transient lactobacilli (L. casei, L. plantarum, L. brevis, L. buchneri and L. lactis) were found simultaneously. Within the Lactobacillus species L. acidophilus type I, L. fermenti type IVa and L. salivarius type Ia were most frequently found in the feces of all age groups. In addition, L. acidophilus type X was also often found in the feces of senile persons. In connection with the numbers of species or types of lactobacilli in one fecal specimen, one or two species or types were demonstrated in 94,5% of positive cases of infants. With increasing age the numbers increased, especially more than three species or types were demonstrated in 85% of positive cases. In infants in 3 different clinics, clinic-specificities were demonstrated in relation to the species or types of lactobacilli isolated: in each clinic clearly certain species or types of lactobacilli were present. The results indicate that the composition of the Lactobacillus flora in the feces is influenced by lactobacilli orally ingested.