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Using electron probe microanalysis, the authors determined the calcium and magnesium contents in the incisors of rats treated with different doses of tetracycline and oxytetracycline, and in those of untreated rats. The contents of these elements were significantly lower in the dentine of the treated animals than in that of the control animals, whereas the enamel samples showed no statistically significant differences. The concentration tendency in different tooth sections, from the periphery to the pulp canal, i.e., decrease in calcium and increase in magnesium, was not influenced.