Ramseier, Christoph A., Warnakulasuriya, Saman, Needleman, Ian G., Gallagher, Jennifer E., Lahtinen, Aira, and Ko-Autoren des 2 European Workshop's Position Papers†
Müller BM, Rosskopf F, Paper DH, Kraus J, and Franz G
Subjects
Animals, Antineoplastic Agents, Phytogenic analysis, Antineoplastic Agents, Phytogenic pharmacology, Cell Survival drug effects, Female, Hydrolysis, Immunologic Factors pharmacology, In Vitro Techniques, Limulus Test, Lymphocytes drug effects, Macrophages drug effects, Magnetic Resonance Spectroscopy, Male, Methylation, Mice, Mice, Inbred C3H, Mitogens pharmacology, Phagocytosis drug effects, Plant Extracts chemistry, Plant Extracts pharmacology, Polysaccharides pharmacology, Protease Inhibitors pharmacology, Structure-Activity Relationship, Tumor Cells, Cultured drug effects, Plants, Medicinal chemistry, Polysaccharides chemistry
Abstract
A water extraction of crushed leaves of Nerium oleander yielded 2.3% of a crude polysaccharide. The main fraction (67%) represents a pectic polysaccharide mainly composed of galacturonic acid besides rhamnose, arabinose and galactose. The polysaccharide structure was characterized by NMR, mild acid- and pectinase treatment combined with GC-MS analyses. In vivo tests for a possible antitumor activity did not result in a significant action. Investigation of immunomodulating activity brought some indications for mitogenic activity and a weak macrophage-mediated cytotoxicity. Increase of phagocytosis could not be doubtlessly assigned to the polysaccharide due to the high amount of endotoxin in the homogenous fraction OLE 2.
Published
1991
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