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The global pandemic of coronavirus disease 2019 (COVID-19) has posed a serious threat to human society on both perspectives of health and economy. Ultraviolet (UV) has been verified capable to damage the DNA and RNA of various microorganisms, including the new coronavirus. The parabolic reflector, a key component of the upper-room ultraviolet germicidal irradiation (UVGI) device, plays a vital role in collating and restraining ultraviolet light. In order to deeply understand the influence of the reflector in the device on the spatial distribution and numerical value of the ultraviolet radiation intensity, we measured and simulated the efficacy of the reflector equipped with a single-tube UV lamp. At a distance of 50cm from the reflector, a virtual plane with a side length of 40cm×40cm parallel to the exit port was introduced. At room temperature of 20 °C, measured and simulated reflectors were carried out at three heights A, B, and C, which were 25 cm, 20 cm, and 15 cm. We have concluded that as the height of the reflector increases, the irradiance value in the space also increases; then decreasing the height of the reflector is more conducive to the uniform distribution of the irradiance in the space. In conclusion, exploring the appropriate reflector height to obtain the balance of UV intensity and distribution is meaningful for improving the efficiency of the upper layer device