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Objective: To observe the effects of Grifola frondosa polysaccharides (GFP) on proliferation, apoptosis, cell cycle, expression of cyclin and apoptotic proteins of human hepatocellular carcinoma HepG2 cells, and to explore the mechanism of its inhibition on proliferation of HepG2 cells. Methods: The experiment was divided into control group and PGF group. Different concentrations of PGF solution were used to interfere with human hepatocellular carcinoma cells HepG2 in vitro. MTT assay was used to detect the effects of different concentrations of PGF on the survival of HepG2 cells. The apoptosis rate and cell cycle distribution of HepG2 cells induced by PGF were detected by flow cytometry. The expressions of Bcl-2, Bax and Caspase3, Cyclin-A1 and Cyclin-B1 were detected by immunohistochemistry. Results: Compared with the control group, PGF could effectively inhibit the proliferation of human hepatoma HepG2 in a concentration-dependent manner. Cell cycle detection showed that the proportion of S phase in each group was 24.71%, 28.78%, 36.26 and 42.39%, respectively, indicating that cells were blocked in S phase. The immunocytochemical results showed that the expression of Cyclin A1 protein decreased significantly, and the expression of cyclin-B1 was not significantly different before and after treatment. Flow cytometry showed that the apoptosis rates in control group and PGF group were 0, 18.0%, 30.5% and 49.5%, respectively. The difference between PGF group and control group was significant. Immunocytochemical results showed that PGF could significantly inhibit the expression of mitochondrial apoptosis inhibitor Bcl-2, increase the expression of pro-apoptotic factor Bax in a concentration-dependent manner, and up-regulate the percentage of Bcl-2/Bax to induce apoptosis of hepatocellular carcinoma cells. Conclusion: PGF can inhibit the proliferation of human hepatoma HepG2 cells by inducing apoptosis, inhibiting cell cycle arrest and expression of related cyclin and apoptotic proteins.

The mental health of adolescents is a hot topic of discussion in today's society. Researchers have found that family environment and atmosphere have an impact on the emotional, cognitive, and behavioral development of adolescents. At present, the research on the impact of parental upbringing on adolescent psychological resilience is not clear and further exploration is needed. The research topic of this article is the impact of parental upbringing on the psychological resilience of adolescents. The research method is as follows. Firstly, this study collected questionnaire survey data on adolescent psychological resilience and parental parenting styles. Secondly, this study conducted reliability and validity analysis, descriptive statistics, and correlation analysis on the data to understand the correlation between adolescent psychological resilience and parental parenting styles. Research has found that emotional warmth and family support factors in parenting style are positively correlated with psychological resilience. Refusal and emotional control factors are negatively correlated with adolescent psychological resilience. Therefore, parents should try to adopt positive parenting methods to avoid excessive control, punishment, and rejection, and thus more effectively cultivate the psychological resilience of adolescents.

The relationship between Japan and Korea has complex historical, political, and cultural dynamics, often characterized by tensions and disputes. Influenced by the intervention of the superpowers, these two countries are economically interdependent and politically opposed. Despite the efforts of the Korean leader to improve the trend, the bilateral relationship faces challenges due to unresolved factors such as historical issues, territorial disputes, and war reparations. This paper takes the external threats affecting the cooperation between Japan and Korea as the research object and evaluates the current situation and obstacles to the bilateral relations between Japan and the Republic of Korea by analyzing the impact of actions taken by countries other than Japan and Korea on their domestic governance, economic conditions, foreign policy decisions, military arrangements, and bilateral relations development. Also, this paper prospects favorable conditions for tightening further bilateral communication, and looks forward to the possibilities of future cooperation between the two countries.

Hannah Arendt focused on the connection and distinction between Labor, Work and Action in “Human Condition”. This article is based on the philosophical situation to explore the political situation and the human situation, taking the research of the core theory as the entry point, focusing on Arendt’s analysis and views on human characteristics in order to conclude my personal opinion.

In this study, the young leaves of Cassia mimosoides were used as explants. On the basis of 1/2 MS +2.0 mg/L 6-BA +0.5 mg/L NAA medium, we observed the effect of adding different factors on the callus induction of C. mimosoides. The results shown that after 20 days of callus induction, it was found that the addition of 150 mg/L vitamin C (VC) did not affect the induction efficiency, but the growth of callus became slower; and when the concentration was higher than 150 mg/L, the formation of callus would be inhibited. The induction of callus would be restrained while adding different concentrations of Cu2+ into mediums. Moreover, glutamine (Gln) had little effect on the induction of callus. Furthermore, the addition of hydrolyzed casein (CH) would not affect the formation of callus, but the high concentration of CH could affect the growth status of callus. The induction efficiency of callus was severely inhibited by exogenous addition of silver nitrate (AgNO3), and the growth status of callus was poor, and the phenomenons of early rooting had happened at the same time. The results of this study provided a theoretical basis for the subsequent optimization of bud proliferation and rooting in C. mimosoides.

Chip On Film(COF) is the key component of electronic products, and is different from Printed Circuit Board(PCB). The properties of high flexibility, thin thickness, lightweight and high wiring density make it difficult to inspect COF, especially dust particles interference. Dust particles are similar to defects, and it is hard to identify dust particles from defects, so dust particles interference of quality test is the difficulty of automatic surface defect detection. In this paper, a new method to identify dust particles is discussed from abnormal area called junction points detection and machine learning method Support Vector Machine(SVM) according to the characteristics of dust particles. As a result, a 94.8% correct rate of dust particles images identification has been achieved with the method.