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Anaerobic continuous stirring tank reactor (CSTR) was employed for biohydrogen production by anaerobic co-digestion from the mixture of herbal wastewater (HW) and monosodium glutamate wastewater (MGW). A series of blend volumetric proportions (MGW/HW), organic loading rates (OLR) were used as control strategy and evaluated for optimal biohydrogen production. The highest biohydrogen production of 7260±7.7 mL L-1 d-1 was attained at blend proportion of 15%, OLR of 41 g COD L-1 d-1 while the highest biohydrogen yield of 508.9±7.2 mL g CODremoved-1 was observed at same conditions. This study demonstrated the feasibility and effectiveness of enhanced biohydrogen production by adding MGW to compensate the low nitrogen content of HW.