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In this study, the testing time was reduced to 1 h through optimizing, and an LOD of 0.3±0.15 μg·L-1 and an IC50 of 2.7±0.3 μg·L-1 were achieved by the optimized CD-ELISA. The sample extraction of 1-fold acetonitrile followed by a 20-fold dilution in assay diluted buffer has proven to be sufficient to eliminate the influence of matrix. The CD-ELISA was validated further by comparing with the standard HPLC. The average recovery ratio of 89.7%~112.7% was obtained, and the coefficients of variation were less than 11.0%. In actual samples detection, except for Pleurotus eryngii, carbendazim was not detected, and other mushrooms were all detected, and only carbendazim in Volvariella volvacea exceeded the standard, and the over-standard rate was 16.7 %. In a word, the rapid, sensitive and efficient CD-ELISA for quantifing carbendazim in mushrooms was established in the study.

In this paper, the habitat of Cerasus clarofolia was investigated and analysed, under natural conditions the growth of Cerasus clarofolia is influenced by low temperature of winter. The seeds of Cerasus clarofolia need low-temperature to germination. The seedling with 4 leaves grew well after introduction, the survival rate reached 79.7%. Planting with clear water was found easier to take roots, the roots were come from the lenticels, under diffent environmental conditions, the lenticels formed morphological, physiological differences are the important factors take roots for Cerasus.