Search

The article presents the results of two-year studies on the biological effectiveness of biofungicide Serenade, ASO, SC (Bacillus amyloliquefaciens, strain QST-713, titer not less than 1x10 9 CFU/ml) in the fight against bacterial black spot on the leaves and fruits of indoor tomatoes in the conditions of the II soil-climatic zone of the Crimea. It was found that a five-time treatment with biofungicide Serenade, ASO, SC, the rate of application of 5.0, 6.5 and 8.0 l/ha, contributed to a decrease in the intensity of development of Xanthomonas campestris pv. vesicatoria on leaves by 2.3-2.9 times, on fruits by 2.2-2.7 times. On the 10th day after the fifth spraying, the biological effectiveness of biofungicide Serenade, ASO, SC in the application rates of 5.0, 6.5 and 8.0 l/ha, with an average development of bacterial black spot on the control variant (3.3-5.2% on leaves, 2.9-3.3% on fruits), on leaves was 76.5-85.1%, on fruits – 71.6-75.0%. On the day of the last harvest, on the 29th day after the last treatment, the biological efficiency on leaves was 57.3-65.4%, on fruits – 54.5-62.6%. The difference of five-time treatment with biofungicide Serenade, ASO, SC in the norm of application of 8.0 l/ha, with the standard is insignificant, within the limits of experimental error. The positive effect of biofungicide Serenade, ASO, SC on yield was noted. The difference with the control (77.9 c/ha) is 34.9, 36.9 and 41.0 c/ha.

At the present stage of science development it is necessary to develop and implement the grape breeding programs for immunity basing on the international level of knowledge about the genetics of immunity to pathogens that cause culture diseases. The extensive material on genes of grape resistance to Plasmopara viticola and Erysiphe necator (Uncinula necator), which cause mildew and oidium diseases, has been collected thanks to the MAS technology. There is an evidence of necessity to pyramidize genes of resistance to these pathogens in one progeny genome for consistent field resistance to the complex of these pathogens. However, even at present, the issue of study the transfer of specific genes of resistance from parents to the progeny, their combining ability in one genome, and gene expression during pathogenesis remains relevant. This publication discusses the formation of grape breeding program in the FSBSI Institute Magarach of the RAS on introgression of resistance genes of the species Vitis rotundifolia (Muscadinia rotundifolia), and also presents the results of phytopathological screening of populations resulting from crossing with this species.

Viral infections cause the reduction of yields and efficiency of vine cultivation in all viticultural regions of the world. Simultaneous infecting with several viruses, the so-called mixed infection, can significantly increase the severity of symptoms. The article presents the results of study of mixed infecting of grapevine with viruses in the vineyards of the Crimean Peninsula during 2011-2020. More than 800 grapevine samples of 153 varieties selected in 23 vineyards of the Crimea were analyzed over the entire period of study. Presence of phytopathogenic viruses in the samples was determined by RT-PCR using virus specific primers. The share of 25.5 % of all the samples tested gave positive result for one or more of the following viruses: GFLV, GLRaV-1, GLRV-2, GLRaV-3, GFkV, GVA, GRSPaV, ArMV, GFLV. During the process of studyno vines affected by GVB virus were identified. Single-agent infection was found in 20% of the examined vines. The amount of vines affected by mixed infection was 5.48 % of all tested. In a mixed viral infection more common combinations are: GRSPaV/GFkV; GVA/GRSPaV; GVA/GLRaV-1/GRSPaV; GVA/GLRaV-3/GRSPaV. Vines were infected the most with GRSPaV and GFkV viruses, which is visually manifested in the form of mosaic leaf variegation, leaf chlorosis and the appearance of necrotic spots.

Heat treatment was used as a method of improving the health of grape plants from the phytoplasma. The Binder KBWF 240 growth cabinet was used for thermotherapy, in which plants of the Tsitronny Magaracha variety were cultivated in vitro under simulated conditions with a treatment period of 14 days. The results of repeated testing of samples showed the efficacy of treatment of this grape disease: out of 13 samples of the Tsitronny Magaracha variety, only one revealed the latent form of phytoplasma blackening of grapevine wood Bois noir. To improve the health of grape plants obtained in vitro culture, it is advisable to use the developed technological operations with thermotherapy in the growth cabinet, which allows to rid the planting material of the latent form of phytoplasma associated with blackening of grapevine wood Bois noir.