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In southern Brazil there is a predominance of labrusca and hybrid varieties for wine and juice production due to climatic conditions of high rainfall, temperature and relative humidity. Growing varieties that combine disease resistance and wine quality (PIWI) can be an alternative to improve wine quality. The objective of this work was to evaluate the performance of three PIWI varieties (Felicia, Calardis Blanc and Aromera) in two regions (Videira, 27∘01′S and 51∘08′W, altitude 830 m; and São Joaquim, 28∘13′S and 50∘04′W, altitude 1100 m) in the vintage 2018. The date of occurrence of main phenological stages, productiveindexes, clusters characteristics and grape qualitative indexes was evaluated. There was no difference for budbreak date, but flowering, veraison an maturity time varied between regions. The development of plants is slower when they are cultivated at a higher altitude. The number of clusters per plant and yield were higher at 830 m for all varieties. For productive indexes Felicia and Calardis Blanc varieties stood out in relation to Aromera in all parameters. Among the evaluated varieties, Felicia and Calardis Blanc were better adapted to the lower altitude region and had higher productivity and the same grape quality. On the other hand, Aromera presented higher productivity at 830 m but higher soluble solids content at 1100 m.