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Extruded snacks, typically derived from low-protein corn, can significantly benefit from the incorporation of red beans to boost their protein content and enhance overall nutritional value. In this study, the influence of feed moisture content and extruder barrel temperature on various physical attributes of the extruded products, including moisture content, particle density, bulk density, expansion ratio, and hardness, were examined. The snacks were produced using a SYSLG-IV twin-screw extruder with a throughput capacity of 10-15 kg/h. The ingredient composition remained consistent, consisting of a 20% red bean flour and 80% corn grit blend. Three moisture content levels (14%-18%) and three different extruder barrel temperature settings (120°C-140°C) were used as variables. The results demonstrated that both variables significantly impacted the physical properties of the extruded snacks. Specifically, an increase in feed moisture content or a decrease in barrel temperature led to higher moisture content (1.01%-1.82%), greater particle density (0.17 g/cm3-0.39 g/cm3), increased bulk density (0.12 g/cm3-0.32 g/cm3), greater hardness (8.34 N-32.18 N), and reduced expansion ratio (2.17-3.0).

This study aims to analyse the determinants factors of firm value. company performance is important to the company’s operational performance which is reflected in firm value. The data were collected from 41 manufacturing sector companies in Indonesia. The manufacturing sector has diverse sectors, a very broad scope, and a larger industrial scale The structural equation model-partial least square (SEM-PLS) analysis was conducted to analyse the relationship between variables and test a series of hypotheses. This study reveals the significant impact solvability and profitability have on asset turnover toward the firm value. This study proves that every stakeholder must maintain its existence by maximizing asset turnover to firm value. Conceptually, this study offers asset turnover as a mediating variable of firm value.

Corn is a commonly used raw material for snack production. However, corn has a relatively low protein content, approximately 7%. Consequently, adding soybean flour is expected to enhance the protein content in snacks. This study investigates the impact of moisture content and barrel temperature treatments on the physical properties of the produced extrudates. Snacks were manufactured using a SYSLG-IV twinscrew extruder with a capacity of 10-15 kg/h and a die diameter of 6 mm. The raw material utilized in the experiment comprised a mixture of 20% soybean flour and 80% corn grit. The study involved two main treatments: barrel temperature (at 120°C, 130°C, and 140°C) and moisture content (at 14%, 16%, and 18%). Parameters assessing the physical properties of the extrudates included expansion ratio, hardness, moisture content, water absorption index, and water solubility index. The results demonstrated that increased moisture content led to increased extrudate moisture content (ranging from 1.80% to 4.71%) and hardness (ranging from 17.45 N to 40.16 N). Conversely, it caused a decrease in the expansion ratio (ranging from 2.11 to 2.03), water solubility index (ranging from 8.82% to 7.01%), and water absorption index (ranging from 5.70 to 4.92).

The writing of this paper is motivated by the rising amount of YouTube vloggers and its viewers about learning and introducing one country’s culture virtually. The increasing spread of these cultures is supported by a simple way of seeking out inspiration and things people are interested about, which mainly seek out on YouTube for clearly image. This also includes vlogs where people film their daily life and so their viewers are able to notice and perceive the country or cultural activities they convey. We’re seen that YouTube is prospectively new media for learning in culture and in some way also fosters its vlogger nation culture. The distinction of this research led by Silent Vlogger who unintentionally introduced one country culture. More specifically, the research is analysing Choki’s channel, a Japanese woman who has more than 1 million subscribers. We disclose from her videos, bringing out to the viewers about Japanese culture in interesting and lifeful approach. We also conducted a questionnaire survey to college students in order to maximize the results. The results show that her videos on YouTube is acceptable to be used as new media for promoting as well as learning media of Japanese cultures.

This research aimed to understand the flow of fish commodity, information, and financial in the fish supply chain at the traditional market, through case study in the Beringharjo market Yogyakarta. Data was collected through systematic interview with 18 fish businesspersons and observation on the study site. The study showed fish commodities in the market consisted of marine, freshwater and processed fish (salted/dried fish and soft bone milkfish/bandeng presto). The fish majorly supplied by suppliers from outside Yogyakarta, that reached 86-90% for fresh fish (marine and freshwater) and 100% for salted fish and raw material of bandeng presto. Suppliers and traders in Beringharjo market used flexible methods of payment, such as manual receipt and trust-based relation (for instance pay on other day). The suppliers and traders had been work together for more than five years. The emerging problems were the lack of fresh fish supply and the low quality of processed fish. The supply chain model for fresh fish involved three stages (supplier-seller-ultimate customer/household) and the supply chain model for processed fish in four stages (supplier-wholesaler-trader/seller-ultimate consumer). The supply chain model for the milkfish also consisted of four stages (supplier-fish processor-seller-ultimate consumer). The study indicates the importance of improving local fish production systems to fullfill growing fish consumption in DIY.

The coastal region is a very dynamic region because it undergoes two processes, natural and non-natural. The coastline can change due to waves, tides and human activities. Human activities are mostly carried out in coastal areas including reclamation for ports. Coastal area of Cirebon is one of the areas that experiences land addition or port reclamation which causes changes in the coastline. Images interpretation is one way to find out changes that occur in coastal areas by using Landsat 8 OLI and Landsat 5TM imagery. The image extraction method using RGB 432 can see the differences between the sea-land and how the changes has occurred in the last ten years, by overlaying both of them which results in extensive abrasion and accretion that occurs on the coast of Cirebon. The abrasion area is 107.12 Ha and the accretion is 941.17 Ha.

Drought is water availability that is far below the water needs for life, agriculture, economic activities, and the environment. The impact of severe drought in Indonesia occurred in 2015 due to the strong El Nino phenomenon and positive IOD. The Regional Disaster Management Agency (BPBD) of Karawang Regency noted that drought in 14 villages spread across three subdistricts in Karawang Regency expanded in 2019. One of them is the Ciampel subdistrict. The purpose of this research is to analyze the drought of agricultural land based on green vegetation, soil organic content, and soil moisture using Tasseled Cap Transformation (TCT) method in Ciampel Subdistrict, Karawang Regency in 2015 and 2019. This research uses Landsat 8 OLI imagery in August 2015, September 2015, July 2019, and September 2019. Agricultural land drought includes three indices, namely the brightness index, wetness index and greenness index. Overlay and scoring three drought parameters to making the map drought of agriculture land with four classes such as normal, moderate, high, and very high drought classes. The results show that the drought occurred in 2015 and 2019, but the dry area is more expansive in 2015 than 2019.

Pariaman is a coastal area in West Sumatera which is located near the seismic fracture. The Semangko fracture consists of the Sumatran fault that extends from north to south and the subduction zone of the plate is located at the base where the two Indo-Australian plates meet. This study aims to determines the level of tsunami hazard in Pariaman coastal area. This study is designed based on tsunami inundation model. The analysis results show that the coastal area of Pariaman is at high tsunami hazard with an area of 1032,53 Ha, area that at middle tsunami hazard with an area 160,39 Ha and area that at low tsunami hazard with an area 88,50 Ha.

The development of a city is essentially influenced by the growth and development of the population, where the city as a physical container of urban community activity. Cirebon City is experiencing rapid regional development, such as the access of the Cipali highway which is connected directly to DKI Jakarta (Capital City). Therefore, the city of Cirebon has a relatively high connectivity where the cities cause a movement of people and goods to the city of Cirebon more quickly and more intensely. Since the middle of the 20th century, human activity has been closely linked to global warming that occurred from observations of rising global average temperatures. This study aims to analyze changes in LST in the city of Cirebon. LST was obtained from Landsat images and analysis spatially and temporally. This study also analyzes the effect of NDVI and NDBI with LST in 2015 and 2019 with 93 sample points selected by stratified random sampling and using multiple linear regression methods. Analysis of LST changes is done by overlay method. The analysis showed spatially the city center has a very high temperature. LST changes occur in the city center to the southern part of the city of Cirebon. The results of multiple linear regression tests of land surface temperature are influenced by vegetation density and building density.

Tidal flood is one of the threats to the coastal areas, especially the north coast of Java. The coastal area of Tangerang Regency itself has a history of tidal flood events that occur every year. The tidal flood hazard level can be measured based on the flood characteristic, which includes flood height, flood duration, and flood frequency. Flood characteristic data are obtained by purposive sampling where the tidal flood occurred. Those data are interpolated using Inverse Distance Weighted (IDW) and overlaid to get the tidal flood hazard level in coastal area of Tangerang Regency. The class of non-hazardous dominates Tangerang Regency. The class has 9,727 hectares or 75% of the total coastal area of Tangerang Regency. While the high-class hazard has an area of 1,149 hectares or 9% of the total coastal area of Tangerang Regency. Hazard level may increase in some regions which close to the river mouth. The increasing of hazard level can also be indicated by a low altitude area. Based on the calculation of hazard equilibrium value, the coastal area of Tangerang Regency is dominated by low-class hazard level. There are 12 villages with low-class of hazard level.

Directly or indirectly, goods and services available in natural ecosystems will contribute to human welfare. The human ability to calculate the economic value of ecosystem goods and services is an important thing to do for integrated environmental decision making, sustainable business practices, and land use planning with its geographical scale, and the level of local-social wisdom. For this reason, a comprehensive review and study are needed by analyzing mangrove ecosystem services. Discussions with in-depth descriptive methods are applied to evaluate mangrove ecosystem services specifically, and focus methods and techniques are used for data analysis, and further to understand their potential and disadvantages.

UGM Retention Pond as flood controller has its water supply from Belik River. Based on water quality monitoring results by the Environment Agency (BLH) of DIY in 2012–2016, the water status at the upstream area of Belik river has already contaminated before it flows to the retention pond. This study aims to make UGM Retention Pond not only as flood control, but also as a water treatment. The function can be fulfilled with the innovation of rough filter water type applied to horizontal flow, i.e. Horizontal-flow Roughing Filter. Mass balance analysis on the retention pond was calculated based on the assumption of the pond as a complete-mix reactor with BOD5 of 20.9 mg/L and TSS of 102 mg/L with discharge (Q) of 2.54 m3/s. The maximum target output produced for BOD5 was 3 mg/L and TSS was 50 mg/L so the target of filtering efficiency was 94 %. The analysis results showed the most optimal Horizontal-flow Roughing Filter was elongated beam-shaped design with length dimensions of 44.6 m, width of 2.5 m, and height of 1.7 m. Filter media used in the form of pebble (gravel) with the size of 24–16 mm and assumed to have permeability coefficient k = 0.1 m/s.

Indonesia National Board for Disaster Management (BNPB) said that in 2002-2009 the drought was the second most frequent intensity after the flood disaster. Drought is a condition where a region experiences a lack of water. If this drought occurs in the agricultural area, it will certainly affect the plants that grow. One of the potential detection methods for drought can be using remote sensing. Temperature Vegetation Dryness Index (TVDI) is one method to detect drought potential with two parameters, vegetation index, and temperature. Through the triangle method, there is a relationship between the vegetation index and temperature represented by linear equations. These linear equations are used to calculate TVDI values. This study uses data Landsat 8 image and paddy fields. The objective of this research is how the distribution and the area of drought based on the TVDI algorithm. The TVDI with dry classes diffuse in the central and western parts, it is also the TVDI with the largest area about 155.82 km2 or 44% of the total. TVDI with wet classes can be seen in the western part of the region and the smallest area about 32.94 km2 or 9% of the total.

Kuningan Regency is one of the districts in West Java Province with a high rate of landslide events, especially in the southern part where its hilly landscape is dominated with a steep slope. Due to its vulnerability because of cracked soil after the landslide events, landslide locations in Kuningan Regency might only be safe if surveyed using the unmanned aerial vehicle (UAV). Therefore, in this study, DJI Phantom 4 Pro was flown to capture images of landslide locations with a 10 cm spatial resolution. Image processing was conducted to generate Orthophoto and Digital Surface Model (DSM) to give information about the direction and area of landslides. Two sub-districts where landslides occurred, namely Darma and Selajambe, were chosen for landslide mapping using UAV. Results show that in Darma Sub-district, the landslide area is approximately 7,026 m2, while in Selajambe Sub-district is around 8,699 m2. The study results are very useful to analyze the factors affecting landslide events such as slope.

This article will analyze the spatial pattern as well as the degradation pattern of the coral reefs in the Bunaken National Park. Bunaken National Park is a marine national park located in the Province of North Sulawesi; the park was built as means of conservation as well as providing a region for tourism. The national park contains a different type of marine and land ecosystem, one of the many types of the ecosystem that are in the national park is coral reefs. Coral reefs in Bunaken National Park provides different kinds of function and benefits whether for the marine habitats that live around the ecosystem, as well as for the local people who live in the islands of the national park. Remote sensing could be used as a tool to identify the spatial pattern and the type of ecosystem that habits inside shallow sea water. The main issue with this method is that the research cannot be conduct directly to identify which type of ecosystem specifically (such as coral reefs, seagrass, etc.), as well as its condition. Therefore, data collecting is necessary to observe and identify the ecosystem and its condition specifically. This study uses satellite image from Landsat 8 OLI as the main secondary data to be processed. The satellite image will be processed by using an algorithm of shallow water analysis that was introduced by Lyzenga in 1981. Since data verification and data observation is needed for this study, the research observes the pattern of the different type of ecosystem and its condition that spreads around Bunaken National Park. The verification and observation process was done by GPS, there were 250 different samples from the data that were collected around the Bunaken National Park. The sample that was collected in the study area will be used to classify the satellite image that has been processed by shallow water algorithm, on which could identify: seagrass, bleached coral reefs, deceased coral reefs, and healthy coral reefs around the national park. The results of this study show the spatial pattern of the coral reefs is located usually around the islands in the Bunaken National Park. The results show that the coral reefs are mostly located around the islands in the National Park. The map results show that the healthy coral reefs are usually located in the outermost layer around the shallow water ecosystem. The bleached reefs are usually located in the middle section of the shallow water, between the healthy coral and the islands itself. Most of the reefs that died and bleached are in the southwest of Bunaken Island, and the northwest of Nain Island.

Many countries in the world have various kinds of problems, such as disasters. Indonesia is one of the countries with high area and population affected by landslides, such as in several regions in Indonesia, namely Bogor Districts. The affected population can be caused by the increasing number of residents and built-up areas, as well as the low level of public knowledge about landslides. The high population affected can describe the level of vulnerability and capacity that exists in the community, such as in South Babakan Madang Subdistrict. The density of the population and the built-up area which includes settlements, public facilities and community knowledge related to landslides that are different in 3 Countryside namely Bojong Koneng, Cijayanti, and Karang Tengah make the people in South Babakan Madang Subdistrict vulnerable to landslides. The modified scoring method with reference to Perka BNPB No. 02 of 2012 can be used to determine the level of vulnerability and capacity of the community. The results show that South Babakan Madang Subdistrict is dominated by moderate vulnerability with an index between 0.34 - 0.67. The capacity in the South Babakan Madang Subdistrict tends to be homogeneous in the low capacity class with an index < 0.34.

One of the world’s most spectacular ecosystems in this world is the coral reef. In Indonesia, Bangka Belitung is one province which has beautiful coral reefs and has become one of the tourist attractions. However, there might be a loss of the coral reefs area that can be caused by natural factors and human activities. This study aims to analyze the distribution and the changing of coral reefs that occurred in the islands of tourist destination in Belitung Regency from 2005 to 2018 and to analyze its factors. Landsat satellite imageries used in this study are Landsat 5 TM, Landsat 7 ETM+, and Landsat 8 OLI/TIRS. The distribution of coral reefs will be determined by image data processing. Then, overlay methods are used to analyze the changes and its factors. Based on the analysis, in the year of 2005-2018, there are 3.93 km2 areas of coral reefs that have decreased. On the top of that, there are 1.34 km2 or about 34.04% of coral reefs areas have decreased that caused by non-natural factors. It can be concluded that the decreased of the coral reefs occurred in Belitung tourism destination islands, are still dominated by natural factors rather than a non-natural factor.

Indonesian agricultural system has improved significantly along with technological developments. However, as a result of technological developments, agricultural land becomes narrow. To overcome the increasingly narrow farming land, start developing systems suitable for planting with aeroponic system. Unpredictable environmental climate changes can disrupt productivity growth and create low quality crops. To solve this problem, monitoring system based on Android needed. This system is used to monitor in parameters change, the parameters include temperature and humidity. Android-based monitoring system uses java programming and is open source, besides the user can monitor the development of spinach plants, by looking at data that has been stored into the database so that users can know the development of the plant. This application can be accessed via smartphone with android operating system as a client server.

Landslide is the third largest disaster in Indonesia, including in Probolinggo. Approximately 50% of Probolinggo regency area is a potential landslide region. This area is located in the southern part of Probolinggo regency with hilly terrain and slopes. The number of landslide events in 2015-2016 is recorded 21 events. Potential landslide hazard was analyzed by SINMAP (Stability Index Mapping) method using physic condition, slope, digital elevation model, and rainfall. The risk level of landslide hazard is derived from the crosstab between landslide hazard potential and vulnerability index covering exposure aspect, sensitivity, and adaptive capacity with variables consisting of population density, livelihood, the proportion of the vulnerable population, building quality, and the number of poor households. The high risk of landslide hazard in Probolinggo regency is found in four districts of Lumbang, Pakuniran, and Kotaanyar sub-district. Potential landslide areas are spread in the south of Probolinggo Regency, especially in Krucil subdistrict with 15-40 % slopes. Based on the spread of landslide location, as many as 81% of landslide locations occur in high and medium landslide potential areas.

Bacteria derived from swamp sediments and aquaculture ponds potential to be probiotics. The study aims to determine the sequence of 16S r RNA gene of isolate of probiotics candidate bacteria from sediment of swamp, to determine the phylogenetic tree between the bacterial species from isolates and Gene Bank data central for their potential as probiotic. The samples of bacteria resulted from pure isolation selected from the sediment of pond cultivation and swamp waters at Lebung Karangan Reservation, Ogan Ilir Regency, Indralaya, South Sumatra. This study was started from cultivating bacteria, extracting DNA of bacteria, amplification 16S r RNA genes by PCR, running electrophoresis, and sequencing the amplicon for determining DNA barcodes of bacteria from sediment of swamp and rearing pond. The result of BLAST analysis showed that KA isolate had the highest similarity 97% with Streptomyces sp. Hjorring101 from Denmark and RA isolate had the highest similarity 98% with Streptomyces sp. BD99 from Pakistan. KE isolate had the highest similarity 99% with Bacillus subtilis CESi5 from Japan and RE isolate had the highest similarity 93% with Bacillus sp.2bFR from Manado. All of isolates were bacteria potentially as swamp aquaculture probiotics.

Election of regional-head candidates without suspect as choosing leaders with integrity and quality as well as without legal problems is a realization of democratic election. Empirical facts show that 2018 regional-head election involves some regional heads identified as suspects. Laws needs to be able to answer such facts. Therefore, this study investigates the legal framework of regional-head candidates’ requirements and the development of legal concept of regional-head election without suspect. This study is normative juridical, and secondary legal sources is employed. This study contributes to the development of legal concept of regional-head election without suspect in holding the upcoming 2022 regional-head elections.

Surabaya rapidly developed to the east region which grew the formal/non-formal settlement in Pamurbaya. This area is dominated by fishpond that is converted into settlement area. This research aims to observe the dynamic change of fishpond which is already converted into settlement area in the east coast of Surabaya, during 2004-2017. This research applies landsat image 7 ETM+ and landsat 8 OLI with overlay method which is analyzed descriptively. During 2004-2017, the settlement area had been developing for 218,296 Ha or 88% and during the same years, the fishpond had been reducing for 531,639 Ha or 24%, this reduction can change into mangrove or settlement area. During 13 years, the change of fishpond into settlement area is 15%. The research area which experienced the change is the fishpond of Kejawan Putih (24%), Medokan Ayu area (17%), and Keputih area (16%).

This study aims to discuss the policy of using renewable energy in the form of rare metal eart as an effort to build national energy security. The research method used a legal research looking from various perspectives in social science. Law is seen as a space for the process of scientific study in order to seek truth. The use of relevant legal research wants to understand the law more thoroughly. In performing implementation analysis, using the method of Regulatory Impact Assessment (RIA) with focus on energy regulation. The results of the study show that: First, the policy of the Indonesian republic government regarding the use of new energy and renewable energy aims to prepare the carrying capacity of national energy security. This policy has not fully gone well. The policy is not supported by consistency in issuing derivative policies. Second, the use of new energy and renewable energy, especially rare earth metals as part of efforts to encourage national energy security in Indonesia is still very far from expectations. The use of rare eart metal is only around 0.7% of the use of new energy. Efforts to explore and exploit rare earth metals have not been carried out in a timely manner. Whereas the potential of rare earth metals is a strategic community and has the potential to encourage national energy security in Indonesia. Indonesia is projected to produce rare earth metals reaching 20% of the world's supply.

Citizen participation is the key to democracy. The development of information and communication technologies makes citizens more empowered, have broad access to information and able to influence policy making, especially at the local level. This paper will describe digital democracy in rural indonesia, when the villagers report their local isues and fight for their right to have a favorable policy. “Speaker Kampung” is an online community media that express the aspirations of citizens in Ketangga Village, East Lombok, West Nusa Tenggara, Indonesia. “Speaker Kampung” is a prototype of a digital democracy in Indonesia.

A suspension bridge and a cable-stayed bridge are compared under various loads and environmental conditions in this study. Under live, dead, and seismic loads, the research investigates axial forces, displacements, bending moments, and shear forces using advanced analytical methods and software such as CsiBridge, SAP2000, and Staad Pro. Steel plays a key role in the construction of suspension bridges and cable-stayed bridges, as this study thoroughly compares both types of bridges. Csi Bridge software is utilized in this study to investigate the axial force, displacement, bending moment, and shear strength of these bridges under various loading conditions. Cable-stayed bridges are recognized for their aesthetic benefits and structural efficiency for medium-to-long spans, and suspension bridges for their high tensile strength and durable design. The two bridge types exhibit different shear forces, displacements, and torsional values, underscoring their unique engineering designs.

Industrialization and the attraction of the city have generated urbanization from the villages to the big cities. The downgrading scheme can be seen through the agricultural sector which suffers several serious problems toward the increase of a mass urbanization, ageing society, and the decline of the young farmers’ population. The data of this research is analyzed with qualitative methods using content analysis of the literature studies. The explanation will be provided by analyzing what aspects which could make Workaway projects sustainable, including the cultural exchange form and the tourism attraction aspect. The projects mainly tend to focus in finding the workers using counterurbanization attraction of the tourism magnetism around the place. There are approximately 60 projects which are related to agriculture projects around Japan. The purpose of this research is to identify and explain how Workaway Project in Yufuin can provide the network between the local community and the international society to contribute in helping the local farmers. This research found that the project is helpful in solving part of the problems in Japanese agriculture in rural areas.

Non-exclusive breastfeeding is defined as the provision of food or fluids in addition to drugs, vitamins, and minerals to infants before the age of 6 months. Exclusive breastfeeding for infants is seen to prevent and cure stunting and other forms of malnutrition. Primary Health Care (PHC) Margorejo is the health center with the lowest exclusive breastfeeding coverage in Pati Regency in 2017 and 2018. The purpose of this study was to analyze the factors related to the behavior of non-exclusive breastfeeding in the working area of the PHC Margorejo in Pati Regency. This study uses a case control method. The research sample consisted of 70 consisting of 35 cases and 35 controls. The research targets were mothers who have 6-11 months old infants in the working area of the PHC Margorejo. Bivariate analysis showed a low level of maternal knowledge (p = 0.008), negative maternal attitudes (p = 0.022), and the absence of support from health workers (p = 0.002) related to non-exclusive breastfeeding behavior. Low level knowledge of mothers, negative attitude of mothers, and the absence of support from health workers are all factors related to the behavior of non-exclusive breastfeeding in the working area of PHC Margorejo in Pati Regency.

Leptospirosis, a zoonotic disease, transmitted to human trough contact with contaminated animal urine and contaminated environment. Demak District is an endemic area where cases increased in the past 2 years. The aim of the study was to map environmental risk factor of Leptospirosis and to determine Leptospirosis vulnerable zone using cross-sectional study design. There were 42 cases mapped by GPS and overlaid using remote sensing (Quickbird image) by using ArcView program then interpreted by Spatial Feature and Spatial Analyses. Leptospirosis cases were spread out and grouped in Demak Sub District area. More cases were males (61.9%), 21-50 years old age group (59.3%) and farmers (40.4%). Spatial analyses showed that all the leptospirosis cases took place in the area with low plain

The Gajah Mungkur sub-district in Semarang, Indonesia had highest leptospirosis cases (reported in human with seven infected and one dead) in 2015. The purpose of this study was to analyze the association between house sanitation and density of rats with Leptospira sp. infection in rats. The study design was cross sectional observational analytic. The number of 308 trapswere placed in study sites over three consecutive nights afterwards. Every houses were placed with four traps, inside and outside. Trapped rats were anesthetized with atropine dose from 0.02 to 0.05 mg/kg body weight of rats continued with Ketamine HCL dose of 50-100 mg/kg body weight of rats by injecting in the thick thigh muscle of it. After that, identification of rats by species and gender then continues with surgery in which a kidney sample was taken to confirm the presence of bacteria Leptospirasp using PCR techniques. The trap installed in 77 houses which later had further observation on house sanitation which includes the existence of a pile of used goods, food storage, garbage can, and the presence of the ceiling, windows and other ventilation.Data was analyzed using distribution frequency and bivariate chi-square test. We had 100 rats captured with live traps as the samples.The proportion of Rattusnorvegicuswas 27% (14.8% positive Leptospira sp.infection) and Rattustanezumi 73% (11%positive Leptospira sp.infection). The proportion of male and female rats were almost equal. The statistic test result was significant between the density of rats (p = 0.0001, OR 12.833, 95%CI: 1.565-105.261) and sex of rats (p = 0.019, OR 0.095, 95%CI: 0.012–0.769) with Leptospira sp. infection in rats. The number of rats may increase the infection of Leptospirasp., especially female rats and poor condition of house sanitation. It is recommended to improve house sanitation and regularly trapping rats.

Previous studies mostly defined Tembalang as an endemic area of dengue fever with highest incidence rate (IR) in District of Semarang. The incidence of dengue fever in Tembalang in 2016 is still high, which is 113.9 per 100.000 citizens. A number of free larvae in Tembalang is still below the target (86.26%). The presence of Aedes sp larvae is influenced by human and environmental factors. The aim of the study was to identify the relation between container characteristic, practices of mosquito habitat diminishing, and the ownership status of residence with the presence of Aedes sp larvae in Tembalang. This research was an analytic observational research with stratified random sampling. Data collection methods were observation and interview, conducted from August-October 2017. The result of this study showed that House Index (HI) was 25%, Container Index (CI) was 11.2%, Breteu Index (BI) was 26% and number of free larvae was 75%. Chi-square analysis showed number of container was related to the presence of Aedes sp larvae (p 0.000). The society needs to make efforts to improve the number of free larvae by doing practices of mosquito’s habitat diminishing especially if they own more than 3 containers.